Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/15346
Tipo do documento: Artigo
Título: Molecular characterization of hepatitis A virus isolates from Goiânia, Goiás, Brazil
Autor: Fiaccadori, Fabíola Souza
Pereira, Maristela
Coelho, Alexandre Siqueira Guedes
Borges, Ana Maria Tavares
Rocha, Juliana Alves Parente
Soares, Célia Maria de Almeida
Cardoso, Divina das Dôres de Paula
Abstract: Hepatitis A virus (HAV) infection is a public health problem worldwide and the virus has been classified into six genotypes. In Brazil, the only genotype that has been found is genotype I, predominately from subgenotype IA. Here, the HAV genotypes were analyzed of 18 isolates circulating between 1996-2001 in Goiânia, state of Goiás, Brazil. Viral RNA was extracted from 18 serum samples and amplified (RT-PCR/nested-PCR), followed by the genomic sequencing of the VP1/2A junction region of the HAV genome. Sequences of 168 nucleotides were compared and analyzed using the BLAST N, Clustal X and PAUP v. 4.10b programs. All samples were classified as genotype I, with 10 belonging to subgenotype IA and eight to subgenotype IB. The subgenotype IA isolates showed greater diversity than the subgenotype IB isolates at the nucleotide level. Elevated identity values were found between isolates ob- tained in this study and those from other regions of the world, including Brazil, highlighting the high conservation among different isolates of this virus. However, changes in the HAV subgenotype circulation could also be observed during the evaluated period.
Palavras-chave: Acute hepatitis
Subgenotype IB
Nucleotide sequencing
Subgenotype IA
País: Brasil
Unidade acadêmica: Instituto de Ciências Biológicas - ICB (RG)
Citação: FIACCADORI, Fabíola Souza; PEREIRA, Maristela ; COELHO, Alexandre Siqueira Guedes; BORGES, Ana Maria Tavares; PARENTE, Juliana Alves; SOARES, Célia Maria de Almeida; CARDOSO, Divina das Dores de Paula. Molecular characterization of hepatitis A virus isolates from Goiânia, Goiás, Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 103, n. 8, p. 831-835, Dec. 2008.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1590/S0074-02762008000800015
Identificador do documento: 10.1590/S0074-02762008000800015
URI: http://repositorio.bc.ufg.br/handle/ri/15346
Data de publicação: Dez-2008
Aparece nas coleções:IPTSP - Artigos publicados em periódicos

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