Use este identificador para citar ou linkar para este item:
Tipo do documento: Artigo
Título: Screening for glycosylphosphatidylinositol- anchored proteins in the Paracoccidioides brasiliensis transcriptome
Autor: Ragagnin, Nadya da Silva Castro
Maia, Zilma Alves
Pereira, Maristela
Soares, Célia Maria de Almeida
Abstract: Open reading frames in the transcriptome of Paracoc- cidioides brasiliensis were screened for potential glycosylphosphati- dylinositol (GPI)-anchored proteins, which are a functionally and struc- turally diverse family of post-translationally modified molecules found in a variety of eukaryotic cells. Numerous studies have demonstrated that various GPI anchor sequences can affect the localization of these pro- teins in the plasma membrane or the cell wall. The GPI anchor core is produced in the endoplasmic reticulum by sequential addition of monosac- charides and phospho-ethanolamine to phosphatidylinositol. The com- plete GPI anchor is post-translationally attached to the protein carboxyl- terminus by GPI transamidases. Removal of this GPI lipid moiety by phospholipases generates a soluble form of the protein. The identifica- tion of putative GPI-attached proteins in the P. brasiliensis transcrip- tome was based on the following criteria: the presence of an N-terminal signal peptide for secretion and a hydrophobic region in the C-terminus presenting the GPI-attachment site. The proteins that were identified were in several functional categories: i) eight proteins were predicted to be enzymes (Gel1, Gel2, Gel3, α-amylase, aspartic proteinase, Cu-Zn SOD, DFG5, PLB); ii) Ag2/PRA, ELI-Ag1 and Gel1 are probably sur- face antigens; iii) Crh-like and the GPI-anchored cell wall protein have a putative structural role; iv) ECM33 and Gels (1, 2 and 3) are possibly involved in cell wall biosynthesis, and v) extracellular matrix protein is considered to be an adhesion protein. In addition, eight deduced proteins were predicted to localize in the plasma membrane and six in the cell wall. We also identified proteins involved in the synthesis, attachment and cleaving of the GPI anchor in the P. brasiliensis transcriptome.
Palavras-chave: Paracoccidioides brasiliensis
GPI-anchored proteins
Plasma membrane
Cell wall
País: Brasil
Unidade acadêmica: Instituto de Ciências Biológicas - ICB (RG)
Citação: CASTRO, Nadya da Silva; MAIA, Zilma Alves; PEREIRA, Maristela; SOARES, Célia Maria de Almeida. Screening for glycosylphophatidylinositol (GPI)-anchored proteins in Paracoccidioides brasiliensis. Genetics and Molecular Research, Ribeirão Preto, v. 4, n. 2, p. 326-345, June 2005.
Tipo de acesso: Acesso Aberto
Data de publicação: 2005
Aparece nas coleções:IPTSP - Artigos publicados em periódicos

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
Artigo - Nadya da Silva Castro Ragagnin - 2005.pdf616,28 kBAdobe PDFThumbnail

Este item está licenciada sob uma Licença Creative Commons Creative Commons