Genotyping of group A rotavirus samples from Brazilian children by probe hybridization

Resumo

The G genotyping of 74 group A rotavirus samples was done by RNA- DNA hybridization (dot-blot) using oligonucleotide probes for the VP7 gene region of the human rotavirus serotypes/genotypes 1, 2, 3 and 4. Thirty-one samples could be genotyped by dot-blot showing the following results: G1 = 16, G4 = 6, G3 = 5, and G2 = 4. The data show circulation of genotypes G1-G4 and the predominance of G1. The knowledge of genotypes provides important information concerning rotavirus circulation in Central Brazil.

Descrição

Palavras-chave

Rotavirus, G genotyping, VP7, Oligonucleotides

Citação

CARDOSO, D. D. P.; RACZ, M. L.; AZEVEDO, M. S. P.; MARTINS, R. M. B.; Soares, C. M. A. Genotyping of group A rotavirus samples from brazilian children by probe hybridization. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 34, n. 4, p. 471-473, 2001.