Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/15370
Tipo do documento: Artigo
Título: Identification and characterization of expressed retrotransposons in the genome of the Paracoccidioides species complex
Autor: Soares, Marco Aurélio
Araújo, Roberta Amália de Carvalho
Souza, Marjorie Mendes Marini e
Oliveira, Luciana Márcia de
Lima, Leonardo Gomes de
Alves, Viviane de Souza
Felipe, Maria Sueli Soares
Brigido, Marcelo de Macedo
Soares, Célia Maria de Almeida
Silveira Filho, José Franco da
Ruiz, Jeronimo Conceição
Cisalpino, Patrícia Silva
Abstract: Background: Species from the Paracoccidioides complex are thermally dimorphic fungi and the causative agents of paracoccidioidomycosis, a deep fungal infection that is the most prevalent systemic mycosis in Latin America and represents the most important cause of death in immunocompetent individuals with systemic mycosis in Brazil. We previously described the identification of eight new families of DNA transposons in Paracoccidioides genomes. In this work, we aimed to identify potentially active retrotransposons in Paracoccidioides genomes. Results: We identified five different retrotransposon families (four LTR-like and one LINE-like element) in the genomes of three Paracoccidioides isolates. Retrotransposons were present in all of the genomes analyzed. P. brasiliensis and P. lutzii species harbored the same retrotransposon lineages but differed in their copy numbers. In the Pb01, Pb03 and Pb18 genomes, the number of LTR retrotransposons was higher than the number of LINE-like elements, and the LINE-like element RtPc5 was transcribed in Paracoccidioides lutzii (Pb01) but could not be detected in P. brasiliensis (Pb03 and Pb18) by semi-quantitative RT-PCR. Conclusion: Five new potentially active retrotransposons have been identified in the genomic assemblies of the Paracoccidioides species complex using a combined computational and experimental approach. The distribution across the two known species, P. brasiliensis and P. lutzii, and phylogenetics analysis indicate that these elements could have been acquired before speciation occurred. The presence of active retrotransposons in the genome may have implications regarding the evolution and genetic diversification of the Paracoccidioides genus.
País: Gra-bretanha
Unidade acadêmica: Instituto de Ciências Biológicas - ICB (RG)
Citação: SOARES, Marco Aurélio; ARAÚJO, Roberta Amália de Carvalho; MARINI, Marjorie Mendes; OLIVEIRA, Luciana Márcia de; LIMA, Leonardo Gomes de; ALVES, Viviane de Souza; FELIPE, Maria Sueli Soares; BRIGIDO, Marcelo Macedo; SOARES, Celia Maria de Almeida; SILVEIRA, Jose Franco da; RUIZ, Jeronimo Conceição; CISALPINO, Patrícia Silva. Identification and characterization of expressed retrotransposons in the genome of the Paracoccidioides species complex. BMC Genomics, London, v. 16, p. 376, 2015.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1186/s12864-015-1564-7
Identificador do documento: 10.1186/s12864-015-1564-7
URI: http://repositorio.bc.ufg.br/handle/ri/15370
Data de publicação: 2015
Aparece nas coleções:IPTSP - Artigos publicados em periódicos

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