Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/15377
Tipo do documento: Artigo
Título: Predicting the proteins of angomonas deanei, strigomonas culicis and their respective endosymbionts reveals new aspects of the trypanosomatidae family
Autor: Motta, Maria Cristina Machado
Martins, Allan Cezar de Azevedo
Souza, Silvana Sant'Anna de
Preta, Carolina Moura Costa Catta
Silva, Rosane
Klein, Cecilia Coimbra
Almeida, Luiz Gonzaga Paula de
Cunha, Oberdan de Lima
Ciapina, Luciane Prioli
Brocchi, Marcelo
Colabardini, Ana Cristina
Lima, Bruna de Araujo
Machado, Carlos Renato
Soares, Célia Maria de Almeida
Probst, Christian Macagnan
Menezes, Claudia Beatriz Afonso de
Thompson, Claudia Elizabeth
Bartholomeu, Daniella Castanheira
Gradia, Daniela Fiori
Pavoni, Daniela Parada
Grisard, Edmundo Carlos
Fantinatti-Garboggini, Fabiana
Marchini, Fabricio Klerynton
Luiz, Gabriela Flávia Rodrigues
Wagner, Glauber
Goldman, Gustavo Henrique
Fietto, Juliana Lopes Rangel
Elias-Sabbaga, Maria Carolina Quartim Barbosa
Goldman, Maria Helena de Souza
Sagot, Marie-France
Pereira, Maristela
Stoco, Patrícia Hermes
Mendonça Neto, Rondon Pessoa de
Teixeira, Santuza Maria Ribeiro
Maciel, Talles Eduardo Ferreira
Mendes, Tiago Antônio de Oliveira
Urmenyi, Turan Peter
Souza, Wanderley de
Schenkman, Sergio
Vasconcelos, Ana Tereza Ribeiro de
Abstract: Endosymbiont-bearing trypanosomatids have been considered excellent models for the study of cell evolution because the host protozoan co-evolves with an intracellular bacterium in a mutualistic relationship. Such protozoa inhabit a single invertebrate host during their entire life cycle and exhibit special characteristics that group them in a particular phylogenetic cluster of the Trypanosomatidae family, thus classified as monoxenics. In an effort to better understand such symbiotic association, we used DNA pyrosequencing and a reference-guided assembly to generate reads that predicted 16,960 and 12,162 open reading frames (ORFs) in two symbiont-bearing trypanosomatids, Angomonas deanei (previously named as Crithidia deanei) and Strigomonas culicis (first known as Blastocrithidia culicis), respectively. Identification of each ORF was based primarily on TriTrypDB using tblastn, and each ORF was confirmed by employing getorf from EMBOSS and Newbler 2.6 when necessary. The monoxenic organisms revealed conserved housekeeping functions when compared to other trypanosomatids, especially compared with Leishmania major. However, major differences were found in ORFs corresponding to the cytoskeleton, the kinetoplast, and the paraflagellar structure. The monoxenic organisms also contain a large number of genes for cytosolic calpain-like and surface gp63 metalloproteases and a reduced number of compartmentalized cysteine proteases in comparison to other TriTryp organisms, reflecting adaptations to the presence of the symbiont. The assembled bacterial endosymbiont sequences exhibit a high A+T content with a total of 787 and 769 ORFs for the Angomonas deanei and Strigomonas culicis endosymbionts, respectively, and indicate that these organisms hold a common ancestor related to the Alcaligenaceae family. Importantly, both symbionts contain enzymes that complement essential host cell biosynthetic pathways, such as those for amino acid, lipid and purine/pyrimidine metabolism. These findings increase our understanding of the intricate symbiotic relationship between the bacterium and the trypanosomatid host and provide clues to better understand eukaryotic cell evolution.
País: Estados unidos
Unidade acadêmica: Instituto de Ciências Biológicas - ICB (RG)
Citação: MOTTA, Maria Cristina Machado et al. Predicting the proteins of angomonas deanei, strigomonas culicis and their respective endosymbionts reveals new aspects of the trypanosomatidae family. Plos One, San Francisco, v. 8, n. 4, e60209, Apr. 2013.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1371/journal.pone.0060209
Identificador do documento: 10.1371/journal.pone.0060209
URI: http://repositorio.bc.ufg.br/handle/ri/15377
Data de publicação: Abr-2013
Aparece nas coleções:IPTSP - Artigos publicados em periódicos

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