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Tipo do documento: Artigo
Título: Cell organisation, sulphur metabolism and ion transport-related genes are differentially expressed in Paracoccidioides brasiliensis mycelium and yeast cells
Autor: Andrade, Rosângela Vieira de
Paes, Hugo Costa
Nicola, Andre Moraes
Carvalho, Maria José Albuquerque de
Saltoratto, Ana Lucia Fachin
Cardoso, Renato Sousa
Silva, Simoneide S.
Matos, Larissa Fernandes
Silva, Silvana Petrofeza
Donadi, Eduardo Antônio
Hojo, Elza Tiemi Sakamoto
Passos Júnior, Geraldo Aleixo da Silva
Soares, Célia Maria de Almeida
Brígido, Marcelo de Macedo
Felipe, Maria Sueli Soares
Abstract: Background: Mycelium-to-yeast transition in the human host is essential for pathogenicity by the fungus Paracoccidioides brasiliensis and both cell types are therefore critical to the establishment of paracoccidioidomycosis (PCM), a systemic mycosis endemic to Latin America. The infected population is of about 10 million individuals, 2% of whom will eventually develop the disease. Previously, transcriptome analysis of mycelium and yeast cells resulted in the assembly of 6,022 sequence groups. Gene expression analysis, using both in silico EST subtraction and cDNA microarray, revealed genes that were differential to yeast or mycelium, and we discussed those involved in sugar metabolism. To advance our understanding of molecular mechanisms of dimorphic transition, we performed an extended analysis of gene expression profiles using the methods mentioned above. Results: In this work, continuous data mining revealed 66 new differentially expressed sequences that were MIPS(Munich Information Center for Protein Sequences)-categorised according to the cellular process in which they are presumably involved. Two well represented classes were chosen for further analysis: (i) control of cell organisation – cell wall, membrane and cytoskeleton, whose representatives were hex (encoding for a hexagonal peroxisome protein), bgl (encoding for a 1,3-β-glucosidase) in mycelium cells; and ags (an α-1,3-glucan synthase), cda (a chitin deacetylase) and vrp (a verprolin) in yeast cells; (ii) ion metabolism and transport – two genes putatively implicated in ion transport were confirmed to be highly expressed in mycelium cells – isc and ktp, respectively an iron-sulphur cluster-like protein and a cation transporter; and a putative P-type cation pump (pct) in yeast. Also, several enzymes from the cysteine de novo biosynthesis pathway were shown to be up regulated in the yeast form, including ATP sulphurylase, APS kinase and also PAPS reductase. Conclusion: Taken together, these data show that several genes involved in cell organisation and ion metabolism/ transport are expressed differentially along dimorphic transition. Hyper expression in yeast of the enzymes of sulphur metabolism reinforced that this metabolic pathway could be important for this process. Understanding these changes by functional analysis of such genes may lead to a better understanding of the infective process, thus providing new targets and strategies to control PCM.
País: Gra-bretanha
Unidade acadêmica: Instituto de Ciências Biológicas - ICB (RG)
Citação: ANDRADE, Rosangela V.; PAES, Hugo C.; NICOLA, André M. ; CARVALHO, Maria Jose A. de; FACHIN, Ana Lúcia; CARDOSO, Renato S.; SILVA, Simoneide S.; FERNANDES, Larissa; SILVA, Silvana P.; DONALID, Eduardo A.; SAKAMOTO-HOJO, Elza T.; PASSOS, Geraldo A. S.; SOARES, Célia M. A.; BRÍGIDO, Marcelo M.; FELIPE, Maria Sueli S. Cell organization, sulphur metabolism and ion transport-related genes are differentially expressed in Paracoccidioides brasiliensis mycelium and yeast cells. BMC Genomics, London, v. 7, p. 208, Aug. 2006.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1186/1471-2164-7-208
Identificador do documento: 10.1186/1471-2164-7-208
Data de publicação: 2006
Aparece nas coleções:IPTSP - Artigos publicados em periódicos

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