Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/15401
Tipo do documento: Artigo
Título: Glyceraldehyde-3-Phosphate dehydrogenase of paracoccidioides brasiliensis is a cell surface protein involved in fungal adhesion to extracellular matrix proteins and interaction with cells
Autor: Barbosa, Mônica Santiago
Báo, Sônia Nair
Andreotti, Patrı́cia Ferrari
Faria, Fabricia Paula de
Felipe, Maria Sueli Soares
Feitosa, Luciano dos Santos
Giannini, Maria José Soares Mendes
Soares, Célia Maria de Almeida
Abstract: The pathogenic fungus Paracoccidioides brasiliensis causes paracoccidioidomycosis, a pulmonary mycosis acquired by inhalation of fungal airborne propagules, which may disseminate to several organs and tissues, leading to a severe form of the disease. Adhesion to and invasion of host cells are essential steps involved in the infection and dissemination of pathogens. Furthermore, pathogens use their surface molecules to bind to host extracellular matrix components to establish infection. Here, we report the characterization of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of P. brasiliensis as an adhesin, which can be related to fungus adhesion and invasion. The P. brasiliensis GAPDH was overexpressed in Escherichia coli, and polyclonal antibody against this protein was obtained. By immunoelectron microscopy and Western blot analysis, GAPDH was detected in the cytoplasm and the cell wall of the yeast phase of P. brasiliensis. The recombinant GAPDH was found to bind to fibronectin, laminin, and type I collagen in ligand far-Western blot assays. Of special note, the treatment of P. brasiliensis yeast cells with anti-GAPDH polyclonal antibody and the incubation of pneumocytes with the recombinant protein promoted inhibition of adherence and internalization of P. brasil- iensis to those in vitro-cultured cells. These observations indicate that the cell wall-associated form of the GAPDH in P. brasiliensis could be involved in mediating binding of fungal cells to fibronectin, type I collagen, and laminin, thus contributing to the adhesion of the microorganism to host tissues and to the dissemination of infection.
País: Estados unidos
Unidade acadêmica: Instituto de Ciências Biológicas - ICB (RG)
Citação: BARBOSA, Mônica Santiago ; BAO, Sonia Nair; ANDREOTTI, Patrícia Ferrari; FARIA, Fabricia P.; FELIPE, Maria Sueli S.; FEITOSA, Luciano dos Santos; MENDES-GIANNINI, Maria José Soares; SOARES, Célia Maria de Almeida. Glyceraldehyde-3-Phosphate dehydrogenase of paracoccidioides brasiliensis is a cell surface protein involved in fungal adhesion to extracellular matrix proteins and interaction with cells. Infection and Immunity, Washington, v. 74, n.1, p. 382-389, 2006.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1128/IAI.74.1.382–389.2006
Identificador do documento: 10.1128/IAI.74.1.382–389.2006
URI: http://repositorio.bc.ufg.br/handle/ri/15401
Data de publicação: Jan-2006
Aparece nas coleções:IPTSP - Artigos publicados em periódicos

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