Clinical data and molecular analysis of Mycobacterium tuberculosis isolates from drug-resistant tuberculosis patients in Goiás, Brazil
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Data
2011
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Resumo
Drug resistance is one of the major concerns regarding tuberculosis (TB) infection worldwide because it hampers
control of the disease. Understanding the underlying mechanisms responsible for drug resistance development
is of the highest importance. To investigate clinical data from drug-resistant TB patients at the Tropical Diseases
Hospital, Goiás (GO), Brazil and to evaluate the molecular basis of rifampin (R) and isoniazid (H) resistance in Mycobacterium
tuberculosis. Drug susceptibility testing was performed on 124 isolates from 100 patients and 24 isolates
displayed resistance to R and/or H. Molecular analysis of drug resistance was performed by partial sequencing
of the rpoB and katG genes and analysis of the inhA promoter region. Similarity analysis of isolates was performed
by 15 loci mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) typing. The molecular
basis of drug resistance among the 24 isolates from 16 patients was confirmed in 18 isolates. Different susceptibility
profiles among the isolates from the same individual were observed in five patients; using MIRU-VNTR,
we have shown that those isolates were not genetically identical, with differences in one to three loci within the 15
analysed loci. Drug-resistant TB in GO is caused by M. tuberculosis strains with mutations in previously described
sites of known genes and some patients harbour a mixed phenotype infection as a consequence of a single infective
event; however, further and broader investigations are needed to support our findings.
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Palavras-chave
rpoB, MDR-TB, MIRU-VNTR, katG
Citação
ALVES, Sueli Lemes de Ávila et. al. Clinical data and molecular analysis of Mycobacterium tuberculosis isolates from drug-resistant tuberculosis patients in Goiás, Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 106, n. 6, p. 655-661, 2011.