Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/15414
Tipo do documento: Artigo
Título: Clinical data and molecular analysis of Mycobacterium tuberculosis isolates from drug-resistant tuberculosis patients in Goiás, Brazil
Autor: Alves, Sueli Lemes de Ávila
Metzker, Fabiana Sarcinelli
Araújo Filho, João Alves de
Junqueira-Kipnis, Ana Paula
Kipnis, André
Abstract: Drug resistance is one of the major concerns regarding tuberculosis (TB) infection worldwide because it hampers control of the disease. Understanding the underlying mechanisms responsible for drug resistance development is of the highest importance. To investigate clinical data from drug-resistant TB patients at the Tropical Diseases Hospital, Goiás (GO), Brazil and to evaluate the molecular basis of rifampin (R) and isoniazid (H) resistance in Mycobacterium tuberculosis. Drug susceptibility testing was performed on 124 isolates from 100 patients and 24 isolates displayed resistance to R and/or H. Molecular analysis of drug resistance was performed by partial sequencing of the rpoB and katG genes and analysis of the inhA promoter region. Similarity analysis of isolates was performed by 15 loci mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) typing. The molecular basis of drug resistance among the 24 isolates from 16 patients was confirmed in 18 isolates. Different susceptibility profiles among the isolates from the same individual were observed in five patients; using MIRU-VNTR, we have shown that those isolates were not genetically identical, with differences in one to three loci within the 15 analysed loci. Drug-resistant TB in GO is caused by M. tuberculosis strains with mutations in previously described sites of known genes and some patients harbour a mixed phenotype infection as a consequence of a single infective event; however, further and broader investigations are needed to support our findings.
Palavras-chave: rpoB
MDR-TB
MIRU-VNTR
katG
País: Brasil
Unidade acadêmica: Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
Citação: ALVES, Sueli Lemes de Ávila et. al. Clinical data and molecular analysis of Mycobacterium tuberculosis isolates from drug-resistant tuberculosis patients in Goiás, Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 106, n. 6, p. 655-661, 2011.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1590/S0074-02762011000600003
Identificador do documento: 10.1590/S0074-02762011000600003
URI: http://repositorio.bc.ufg.br/handle/ri/15414
Data de publicação: 2011
Aparece nas coleções:IPTSP - Artigos publicados em periódicos

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
Artigo - Sueli Lemes de Ávila Alves - 2011.pdf342,31 kBAdobe PDFThumbnail
Baixar/Abrir


Este item está licenciada sob uma Licença Creative Commons Creative Commons