Human bocavirus 1 and 3 infection in children with acute gastroenteritis in Brazil
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2012-09
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To determine the positivity rate of human bocavirus (HBoV) 1 and 3 among children who presented with acute
gastroenteritis symptoms during the period of 1994-2004 in the Central-West Region of Brazil, 762 faecal samples
were tested using polymerase chain reaction (PCR) for the detection of HBoV DNA. Primers for a segment of the
non-structural viral protein 1 (NS1) gene of HBoV-1 and HBoV-3 were used. Twelve HBoV-positive samples were
further characterised via genomic sequencing and phylogenetic analysis. Of the samples tested, 5.8% (n = 44) were
positive for HBoV-1 or HBoV-3 and co-infection was observed in 14 (31.8%) of the 44 HBoV-positive samples. Nine
of the 14 samples were also positive for Rotavirus A and five were positive for Aichi virus. The genomic sequencing
of the NS1 partial sequence of 12 HBoV-samples showed that 11 samples were characterised as HBoV-1 and that
one was characterised as HBoV-3. The phylogenetic analysis showed that the HBoV-1 samples had a high sequence
homology to others previously identified in China, Sweden and Brazil. This is the first study conducted in the Cen-
tral-West Region of Brazil to detect HBoV-1 and HBoV-3 in faecal samples from children with acute gastroenteritis.
Further studies are required to define the role of HBoVs as aetiological agents of gastroenteritis.
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Human bocavirus, Brazil, Acute gastroenteritis, Molecular characterisation
Citação
SOUSA, Teresinha Teixeira de; SOUZA, Menira; FIACCADORI, Fabíola Souza; BORGES, Ana Maria Tavares; COSTA, Paulo Sucasas da; CARDOSO, Divina das Dôres de Paula. Human bocavirus 1 and 3 infection in children with acute gastroenteritis in Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 107, n. 6, p. 800-804, Sept. 2012.