Gastroenteric virus detection in fecal samples from women in Goiânia, state of Goiás, Brazil
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2010-06
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Introduction: This was a prospective study that included women seen in the obstetrics and
gynecology sector of Hospital das Clínicas, Federal University of Goiás, in Goiânia, State
of Goiás, with the aim of detecting rotaviruses, adenoviruses, caliciviruses and astroviruses.
Eighty-four women participated in the study and from these, 314 fecal samples were collected.
Out of all of the women, 29 were seropositive for HIV and 55 were seronegative, and 45 and 39
were pregnant and non-pregnant, respectively. Methods: Fecal samples were collected from
each woman once every two months over the period from July 2006 to June 2007, and they were
screened for rotaviruses by means of polyacrylamide gel electrophoresis and immunoenzymatic
assays, for caliciviruses and astroviruses by means of RT-PCR and for adenovirus by means
of immunoenzymatic assays. The astroviruses were genotyped using nested PCR. Results:
Among the 84 patients, 19 (22.6%) were positive for either calicivirus (14/19) or astrovirus
(6/19), while one women was positive for both viruses in fecal samples collected on different
occasions. Most of the positive samples were collected during the months of July and August
(astrovirus) and September and October (calicivirus). None of the samples analyzed was
positive for rotavirus or adenovirus. Gastroenteric viruses were detected in 13/19 (68.4%)
of the pregnant women, whether HIV-seropositive or not. Conclusions: The results from the
present study showed that neither pregnancy nor HIV-seropositive status among the women
increased the risk of infection by any of the gastroenteric viruses studied. This study presents
data on gastroenteric virus detection among pregnant and/or HIV-positive women.
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Gastroenteric viruses, Pregnant women, Calicivirus, Astrovirus, HIV-positive
Citação
FERREIRA, Rui Gilberto; BORGES, Ana Maria Tavares; FIACCADORI, Fabiola Souza; SOUZA, Menira Borges de Lima Dias e; SANTOS, Rodrigo Alessandro Togo; CARDOSO, Divina das Dôres de Paula. Gastroenteric virus detection in fecal samples from women in Goiânia, state of Goiás, Brazil. Revista da Sociedade Brasileira de Medicina Tropical, Uberaba, v. 43, n. 3, p. 240-243, May/Jun. 2010.