Leishmania spp parasite isolation through inoculation of patient biopsy macerates in interferon gamma knockout mice
Carregando...
Data
2010-04
Autores
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
Isolation of Leishmania parasite and species identification are important for confirmation and to help define the epidemiology
of the leishmaniasis. Mice are often used to isolate pathogens, but the most common mouse strains are resistant to infection with
parasites from the Leishmania (Viannia) subgenus. In this study we tested the inoculation of interferon gamma knockout (IFNg KO)
mice with biopsy macerates from Leishmania-infected patients to increase the possibility of isolating parasites. Biopsies from twenty
five patients with clinical signs of leishmaniasis were taken and tested for the presence of parasites. Immunohistochemical assay (IHC)
and conventional histopathology detected the parasite in 88% and 83% of the patients, respectively. Leishmania sp. were isolated in
biopsy macerates from 52% of the patients by culture in Grace’s insect medium, but 13% of isolates were lost due to contamination.
Inoculation of macerates in IFNg KO mice provides isolation of parasites in 31.8% of the biopsies. Most isolates belong to L. (Viannia)
subgenus, as confirmed by PCR, except one that belongs to L. (Leishmania) subgenus. Our preliminary results support the use of
IFNg KO mice to improve the possibility to isolate New World Leishmania species.
Descrição
Palavras-chave
Leishmania (Viannia) subgenus, Experimental leishmaniasis, Leishmania isolation, Leishmania identification, Interferon gamma deficient mice, Leishmaniasis
Citação
OLIVEIRA, Milton Adriano Pelli de; PIRES, Alause da Silva; BASTOS, Rosidete Pereira de; LIMA, Glória Maria Collet de Araújo ; PINTO, Sebastião Alves ; PEREIRA, Ledice Inácia de Araújo ; PEREIRA, Ana Joaquina Cohen Serique; ABRAHAMSOHN, Ises de Almeida; DORTA, Miriam Leandro ; RIBEIRO-DIAS, Fátima. Leishmania spp parasite isolation through inoculation of patient biopsy macerates in interferon gamma knockout mice. Revista do Instituto de Medicina Tropical de São Paulo, São Paulo, v. 52, n. 2, p. 83-88, Mar./Apr. 2010.