Dynamics and kinetics of natural killer cell cytotoxicity in human malaria as evaluated by a novel stepwise cytotoxicity assay
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Data
2006-08
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Resumo
Malaria causes important functional alterations of the immune system, but several of them are poorly defined. To evaluate
thoroughly the natural killer cell cytotoxicity in patients with malaria, we developed a technique capable to assess both the
dynamics and the kinetics of the process. For the kinetics assay, human peripheral blood mononuclear cells were previously
incubated with K562 cells and kept in agarose medium, while for the dynamics assay both cells were maintained in suspension.
NK activity from patients with vivax malaria presented a kinetics profile faster than those with falciparum malaria. NK cytotoxicity
positively correlated with parasitemia in falciparum malaria. The dynamics of NK cytotoxicity of healthy individuals was elevated
at the beginning of the process and then significantly decreased. In contrast, malaria patients presented successive peaks of NK
activity. Our results confirmed the occurrence of alteration in NK cell function during malaria, and added new data about the
NK cytotoxicity process.
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Malaria, Natural killer cells, Plasmodium vivax, Plasmodium falciparum, Malária, Citotoxicidade, Plasmodium vivax, Células natural killer, Plasmodium falciparum, Cytotoxicity
Citação
RIBEIRO-DIAS, Fátima; TOSTA, Carlos Eduardo. Dynamics and kinetics of natural killer cell cytotoxicity in human malaria as evaluated by a novel stepwise cytotoxic assay. Revista da Sociedade Brasileira de Medicina Tropical, Uberaba, v. 39, n. 4, p. 357-364, July/Aug. 2006.