Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/15430
Tipo do documento: Artigo
Título: Thioglycollate-elicited murine macrophages are cytotoxic to Mycoplasma arginini-infected YAC-1 tumor cells
Autor: Dias, Fátima Ribeiro
Nascimento, F. R. F.
Barbuto, José Alexandre Marzagão
Timenetsky, Jorge
Negro, Sonia Jancar
Abstract: Macrophages are important components of natural immunity involved in inhibition of tumor growth and destruction of tumor cells. It is known that these cells can be activated for tumoricidal activity by lymphokines and bacterial products. We investigated whether YAC-1 tumor cells infected with Mycoplasma arginini stimulate nitric oxide (NO) release and macrophage cytotoxic activity. Thioglycollate-elic- ited macrophages from male BALB/c mice were co-cultured for 20 h with YAC-1 tumor cells infected or not with Mycoplasma arginini. The cytotoxic activity was evaluated by MTT assay and nitrite levels were determined with the Griess reagent. Thioglycollate-elicited mac- rophages co-cultured with noninfected YAC-1 cells showed low cytotoxic activity (34.7 ± 8.6%) and low production of NO (4.7 ± 3.1 μM NO 2- ). These macrophages co-cultured with mycoplasma-in- fected YAC-1 cells showed significantly higher cytotoxic activity (61.4 ± 9.1%; P<0.05) and higher NO production (48.5 ± 13 μM NO 2- ; P<0.05). Addition of L-NAME (10 mM), an inhibitor of NO synthesis, to these co-cultures reduced the cytotoxic activity to 37.4 ± 2% (P<0.05) and NO production to 3 ± 4 μM NO 2- (P<0.05). The present data show that Mycoplasma arginini is able to induce macro- phage cytotoxic activity and that this activity is partially mediated by NO.
Palavras-chave: Macrophages
Cytotoxicity
Mycoplasma
Tumor cells
Nitric oxide
País: Brasil
Unidade acadêmica: Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
Citação: RIBEIRO-DIAS, F.; RUSSO, M.; NASCIMENTO, F. R. F. ; BARBUTO, J. A. M. ; TIMENETSKY, J.; JANCAR, S . Thioglycollate-elicited murine macrophages are cytotoxic to Mycoplasma arginini-infected YAC-1 tumor cells. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 31, n. 11, p. 1425-1428, Nov. 1998.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1590/S0100-879X1998001100010
Identificador do documento: 10.1590/S0100-879X1998001100010
URI: http://repositorio.bc.ufg.br/handle/ri/15430
Data de publicação: 1998
Aparece nas coleções:IPTSP - Artigos publicados em periódicos

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