Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/15432
Tipo do documento: Artigo
Título: In Vitro metacyclogenesis of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) amazonensis clinical field isolates, as evaluated by morphology, complement resistance, and infectivity to human macrophages
Autor: Silva Junior, Ildefonso Alves da
Morato, Camila Imai
Quixabeira, Valéria Bernadete Leite
Pereira, Ledice Inácia de Araújo
Dorta, Miriam Cristina Leandro
Oliveira, Milton Adriano Pelli de
Horta, Maria de Fátima Martins
Dias, Fátima Ribeiro
Resumo: This study was designed to assess in vitro metacyclogenesis of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) amazonensis clinical field isolates obtained from patient lesions (L. braziliensis IMG3 and PPS6m; L. amazonensis MAB6). Metacyclogenesis was evaluated by different criteria, namely, promastigote size (morphometric analysis and flow cytometry), surface modifications (loss of lectin or monoclonal antibody (mAb) binding, complement resistance), and infectivity to human macrophages. Growth curves were similar for all parasites evaluated. The various features analyzed were expressed in a high percentage of promastigotes at 6th and 10th days of culture and a low percentage at the 2nd day. However, in most isolates, these features, considered as markers of metacyclogenesis, seemed to develop with different time courses, since the percentages of metacyclic forms detected with each technique were usually different. Parasites from 6th or 10th day and those negatively selected with lectin or mAb similarly infected human macrophages. From all isolates analyzed, L. amazonensis PH8 and MAB6 showed the highest and the lowest levels of susceptibility, respectively, to leishmanicidal activity of IFN-γ/LPS-activated macrophages. Our results showed that by using different techniques to evaluate different aspects of metacyclogenesis (morphological and biochemical modifications) different percentages of metacyclic promastigotes can be detected in each isolate culture.
País: Gra-bretanha
Unidade acadêmica: Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
Citação: SILVA JR., Ildefonso Alves da; MORATO, Camila Imai; QUIXABEIRA, Valéria Bernadete Leite; PEREIRA, Ledice Inácia de Araújo; DORTA, Miriam Leandro; OLIVEIRA, Milton Adriano Pelli de; HORTA, Maria Fátima; RIBEIRO-DIAS, Fátima. In Vitro metacyclogenesis of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) amazonensis clinical field isolates, as evaluated by morphology, complement resistance, and infectivity to human macrophages. BioMed Research International, London, v. 2015, p. 1-15, 2015.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1155/2015/393049
Identificador do documento: 10.1155/2015/393049
URI: http://repositorio.bc.ufg.br/handle/ri/15432
Data de publicação: 2015
Aparece nas coleções:IPTSP - Artigos publicados em periódicos

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
Artigo - Ildefonso Alves da Silva Junior - 2015.pdf2,99 MBAdobe PDFThumbnail
Baixar/Abrir


Este item está licenciada sob uma Licença Creative Commons Creative Commons