Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/15435
Tipo do documento: Artigo
Título: Evaluation of an rK39-based immunochromatographic test for the diagnosis of visceral leishmaniasis in human saliva
Autor: Silva, M. R. B. da
Brandão, N. A. A.
Dorta, Miriam Cristina Leandro
Dias, Fátima Ribeiro
Costa, D. L.
Costa, C. H. N.
Oliveira, Milton Adriano Pelli de
Abstract: Visceral leishmaniasis (VL) is a tropical neglected disease endemic in 98 countries and affects more than 58 000 individuals per year. Several serological tests are available for VL diagnosis, including an immunochromatographic (IC) test with the rK39 antigen and finger prick-collected blood, a rapid and low-invasive test. Here, we investigate the possibility to use saliva as a non-invasive source of biological material for the rK39 IC test. Blood samples from 84 patients with suspected VL were screened by the rK39 IC test, and 29 were confirmed as being infected by a positive rK39 IC test and the presence of amastigotes on smears slides or parasite DNA (detected using PCR-RFLP) from bone marrow aspirate. The rK39 IC test using saliva samples was positive for 17 of the 29 confirmed VL cases (58.6%). The amount of Leishmania-specific IgG or total IgG, as evaluated by an immunoenzymatic assay, was higher in the saliva of patients who had rK39 IC test positivity using saliva, whereas the amount of Leishmania-specific IgA or total IgA was similar to the healthy donors. These results suggest that saliva is not an appropriated material for diagnosing VL with this test.
País: Outros
Unidade acadêmica: Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
Citação: SILVA, M. R. B. ; BRANDÃO, N.A. A.; DORTA, Miriam L.; RIBEIRO-DIAS, F.; COSTA, D. L.; COSTA, C. H. N.; Oliveira, Milton A. P. Evaluation of an rK39-based immunochromatographic test for the diagnosis of visceral leishmaniasis in human saliva. Tropical Biomedicine, Kuala Lumpur, v. 32, n. 3, p. 247-256, 2015.
Tipo de acesso: Acesso Aberto
URI: http://repositorio.bc.ufg.br/handle/ri/15435
Data de publicação: 2015
Aparece nas coleções:IPTSP - Artigos publicados em periódicos

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