Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/15453
Tipo do documento: Artigo
Título: A dysflagellar mutant of Leishmania (Viannia) braziliensis isolated from a cutaneous leishmaniasis patient
Autor: Zauli, Rogéria Cristina
Yasunaka, Jenicer Kazumi Umada Yokoyama
Miguel, Danilo Ciccone
Moura, Alexandre Santos de
Pereira, Ledice Inacia de Araújo
Silva Junior, Ildefonso Alves da
Lemes, Lucianna Gonçalves Nepomuceno
Dorta, Miriam Leandro
Oliveira, Milton Aparecido de
Pitaluga, André Nóbrega
Ishikawa, Edna Aoba Yassui
Rodrigues, Juliany Cola Fernandes
Traub-Csekö, Yara Maria
Katzin, Alcira Tania Bijovsky de
Dias, Fátima Ribeiro
Uliana, Silvia Reni Bortolin
Abstract: Background: Parasites of the Leishmania genus alternate between the flagellated extracellular promastigote stage and intracellular amastigotes. Here we report the characterization of a Leishmania isolate, obtained from a cutaneous leishmaniasis patient, which presents peculiar morphological features. Methods: The parasite was cultured in vitro and characterized morphologically using optical and electron microscopy. Identification was performed based on monoclonal antibodies and internal ribosomal spacer typing. In vitro macrophage cultures, murine experimental models and sand fly infections were used to evaluate infectivity in vitro and in vivo. Results: The isolate was identified as Leishmania (Viannia) braziliensis. In the atypical promastigotes grown in culture, a short flagellum surrounded or interrupted by a protuberance of disorganized material was observed. A normal axoneme was present close to the basal body but without elongation much further outside the flagellar pocket. A disorganized swelling at the precocious end of the axoneme coincided with the lack of a paraflagellar rod structure. The isolate was able to infect macrophages in vitro, induce lesions in BALB/c mice and infect Lutzomyia longipalpis. Conclusions: Notwithstanding the lack of an extracellular flagellum, this isolate infects macrophages in vitro and produces lesions when inoculated into mice. Moreover, it is able to colonize phlebotomine sand flies. Considering the importance attributed to the flagellum in the successful infection and survival of Leishmania in the insect midgut and in the invasion of macrophages, these findings may bring new light into the infectious mechanisms of L. (V.) braziliensis.
Palavras-chave: Flagellum
Leishmania
Mutant
Electron microscopy
País: Gra-bretanha
Unidade acadêmica: Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
Citação: ZAULI, Rogéria C.; YOKOYAMA-YASUNAKA, Jenicer KU; MIGUEL, Danilo C.; MOURA, Alexandre S.; PEREIRA, Ledice I. A.; SILVA JR., Ildefonso A da; LEMES, Lucianna G. N.; DORTA, Miriam L.; OLIVEIRA, Milton A. P.; PITALUGA, André N.; ISHIKAWA, Edna A. Y.; RODRIGUES, Juliany C. F.; TRAUB-CSEKO, Yara M.; BIJOVSKY, A. Tania; RIBEIRO-DIAS, Fátima; ULIANA, Silvia R. B. A dysflagellar mutant of Leishmania (Viannia) braziliensis isolated from a cutaneous leishmaniasis patient. Parasites & Vectors, London, v. 5, p. 11-22, 2012.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1186/1756-3305-5-11
Identificador do documento: 10.1186/1756-3305-5-11
URI: http://repositorio.bc.ufg.br/handle/ri/15453
Data de publicação: 2012
Aparece nas coleções:IPTSP - Artigos publicados em periódicos

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