Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/15454
Tipo do documento: Artigo
Título: Ecto-nucleotidase activities of promastigotes from leishmania (viannia) braziliensis relates to parasite infectivity and disease clinical outcome
Autor: Leite, Pauline Martins
Silva, Rodrigo Gomes da
Figueiredo, Amanda Braga de
Serafim, Tiago Donatelli
Tafuri, Wagner Luiz
Souza, Carolina Carvalho de
Moura, Sandra Aparecida Lima de
Fietto, Juliana Lopes Rangel
Melo, Maria Norma
Dias, Fátima Ribeiro
Oliveira, Milton Adriano Pelli de
Rabello, Ana Lúcia Teles
Afonso, Luis Carlos Crocco
Abstract: Background: Leishmania (Viannia) braziliensis has been associated with a broad range of clinical manifestations ranging from a simple cutaneous ulcer to destructive mucosal lesions. Factors leading to this diversity of clinical presentations are not clear, but parasite factors have lately been recognized as important in determining disease progression. Given the fact that the activity of ecto-nucleotidases correlates with parasitism and the development of infection, we evaluated the activity of these enzymes in promastigotes from 23 L. braziliensis isolates as a possible parasite-related factor that could influence the clinical outcome of the disease. Methodology/Principal Findings: Our results show that the isolates differ in their ability to hydrolyze adenine nucleotides. Furthermore, we observed a positive correlation between the time for peak of lesion development in C57BL/6J mice and enzymatic activity and clinical manifestation of the isolate. In addition, we found that L. (V.) braziliensis isolates obtained from mucosal lesions hydrolyze higher amounts of adenine nucleotides than isolates obtained from skin lesions. One isolate with high (PPS6m) and another with low (SSF) ecto-nucleotidase activity were chosen for further studies. Mice inoculated with PPS6m show delayed lesion development and present larger parasite loads than animals inoculated with the SSF isolate. In addition, PPS6m modulates the host immune response by inhibiting dendritic cell activation and NO production by activated J774 macrophages. Finally, we observed that the amastigote forms from PPS6m and SSF isolates present low enzymatic activity that does not interfere with NO production and parasite survival in macrophages. Conclusions/Significance: Our data suggest that ecto-nucleotidases present on the promastigote forms of the parasite may interfere with the establishment of the immune response with consequent impaired ability to control parasite dissemination and this may be an important factor in determining the clinical outcome of leishmaniasis.
País: Estados unidos
Unidade acadêmica: Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
Citação: LEITE, Pauline M.; GOMES, RODRIGO S.; FIGUEIREDO, Amanda B.; SERAFIM, Tiago D.; TAFURI, Wagner L.; SOUZA, Carolina C. de; MOURA, Sandra A. L. ; FIETTO, Juliana L. R.; MELO, Maria N.; RIBEIRO-DIAS, Fátima; OLIVEIRA, Milton A. P.; RABELLO, Ana; AFONSO, Luís C. C. Ecto-nucleotidase activities of promastigotes from leishmania (viannia) braziliensis relates to parasite infectivity and disease clinical outcome. PLoS Neglected Tropical Diseases, San Francisco, v. 6, n. 10, e1850, Oct. 2012.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1371/journal.pntd.0001850
Identificador do documento: 10.1371/journal.pntd.0001850
URI: http://repositorio.bc.ufg.br/handle/ri/15454
Data de publicação: Out-2012
Aparece nas coleções:IPTSP - Artigos publicados em periódicos

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