A comparative study of the organic content of the hemolymph of Schistosoma mansoni-resistant and susceptible strains of Biomphalaria glabrata
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Data
1997-06
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Resumo
The freshwater snail Biomphalaria glabrata is an intermediate host of the trematode Schistosoma
mansoni. However, some strains of B. glabrata are resistant to successful infection by S. mansoni larvae.
The present work examines the profile of organic acids present in S. mansoni-resistant and
-susceptible strains of B. glabrata, in order to determine whether the type of organic acid present is
related to susceptibility. The organic acids were extracted from the hemolymph of two susceptible B.
glabrata strains (PR, Puerto Rico and Ba, Jacobina-Bahia from Brazil), and from the resistant strains
13-16-R1 and 10R2, using solid phase extraction procedures followed by high performance liquid chromatography.
The organic acids obtained were analyzed and identified by comparison with known standards.
Pyruvate, lactate, succinate, malate, fumarate, acetate, propionate, ß-hydroxybutyrate and acetoacetate
were detected in all hemolymph samples. Under standard conditions, the concentration of
each of these substances varied among the strains tested and appeared to be specific for each strain. An
interesting variation was the low concentration of pyruvate in the hemolymph of PR-snails. Only the
concentration of fumarate was consistently different (p≤ 0.05) between resistant and susceptible strains.
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Biomphalaria glabrata, Susceptibility, Host-parasite interaction, Schistosoma mansoni, Organic acids, Resistance
Citação
BEZERRA, José Clecildo Barreto; BECKER, Wilhelm; ZELCK, Ulrike E. A comparative study of the organic content of the hemolymph of Schistosoma mansoni-resistant and susceptible strains of biomphalaria glabrata. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 92, n. 3, p. 421-425, May/Jun. 1997.