Y-STR haplotype diversity and population data for Central Brazil: implications for environmental forensics and paternity testing
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2015-04
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The central region of Brazil was colonized by internal migration of individuals of different origins, who contributed to the genetic diversity existing in this population. This study determined the allele frequencies and haplotype diversity of Y-STRs in Goiás State, Central Brazil, and compared the data obtained with a sample of the Brazilian population, consisting of individuals from the five geographical regions of Brazil. A total of 353 males were typed for 12 Y-chromosome short tandem repeat (Y-STR) markers. We selected
males who had no degree of relatedness, from the five mesoregions of
Goiás State. DNA was extracted from blood samples followed by the
amplification of the 12 Y-chromosome loci. The products were analyzed
to obtain the allele profiles on an ABI3500 automated sequencer using
the Gene Mapper software. Allele frequencies and haplotype diversity
were estimated by direct counting, and gene diversity for each locus
was computed using the Arlequin software. The results are consistent
with the history of miscegenation of the population of Central Brazil, in
which we observed 321 different haplotypes. The average gene diversity
at the 12 loci was 0.645. DYS385b and DYS389I showed the highest
(0.704) and lowest (0.520) genetic diversity values, respectively. The
FST value between the Brazilian and Goiás populations was 0.00951,
showing no statistical significance. The results of this study allowed
the establishment of haplotypes found in the forensic samples of Goiás
State serving as a reference in the elucidation of criminal cases and
paternity tests, as well as population and evolutionary inferences.
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Y chromosome, STR markers, Haplotype diversity
Citação
VIEIRA, T. C.; GIGONZAC, M. A. D.; SILVA, D. M.; RODOVALHO, R. G.; SANTOS, G.S.; CRUZ, A. D. da. Y-STR haplotype diversity and population data for Central Brazil: implications for environmental forensics and paternity testing. Genetics and Molecular Research, Ribeirão Preto, v. 13, n. 2, p. 3404-3410, Apr. 2014.