Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/15555
Tipo do documento: Artigo
Título: Testing of four Leishmania vaccine candidates in a mouse model of infection with Leishmania (Viannia) braziliensis, the main causative agent of cutaneous leishmaniasis in the New World
Autor: Salay, Gerson
Dorta, Miriam Cristina Leandro
Santos, N. M.
Mortara, Renato Arruda
Brodskyn, Claudia Ida
Oliveira, Camila Indiani de
Mestriner, Clara Lúcia Barbiéri
Rodrigues, Mauricio Martins
Abstract: We evaluated whether four recombinant antigens previously used for vaccination against experimental infection with Leishmania (Leishmania) major could also induce protective immunity against a challenge with Leishmania (Viannia) braziliensis, the species responsible for 90% of the 28,712 annual cases of cutaneous and mucocutaneous leishmaniasis recorded in Brazil during the year of 2004. Initially, we isolated the homolog genes encoding four L. (V.) braziliensis antigens: (i) homologue of receptor for activated C kinase, (ii) thiol-specific antioxidant, (iii) Leishmania elongation and initiation factor, and (iv) L. (L.) major stress- inducible protein 1. At the deduced amino acid level, all four open reading frames had a high degree of identity with the previously described genes of L. (L.) major being expressed on promastigotes and amastigotes of L. (V.) braziliensis. These genes were inserted into the vector pcDNA3 or expressed as bacterial recombinant proteins. After immunization with recombinant plasmids or proteins, BALB/c mice generated specific antibody or cell-mediated immune responses (gamma interferon production). After an intradermal challenge with L. (V.) braziliensis infective promastigotes, no significant reduction on the lesions was detected. We conclude that the protective immunity afforded by these four vaccine candidates against experimental cutaneous leishman- iasis caused by L. (L.) major could not be reproduced against a challenge with L. (V.) braziliensis. Although negative, we consider our results important since they suggest that studies aimed at the development of an effective vaccine against L. (V.) braziliensis, the main causative agent of cutaneous leishmaniasis in the New World, should be redirected toward distinct antigens or different vaccination strategies.
País: Estados unidos
Unidade acadêmica: Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
Citação: SALAY, G.; DORTA, M. L.; SANTOS, N. M.; MORTARA, R. A.; BRODSKIN, C.; BARBIERI, C. L.; RODRIGUES, M. M. Testing of four Leishmania vaccine candidates in a mouse model of infection with Leishmania (Viannia) braziliensis, the main causative agent of cutaneous leishmaniasis in the New World. Clinical and Vaccine Immunology, Washington, v. 14, n. 9, p. 1173-81, Sept. 2007.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1128/CVI.00060-07
Identificador do documento: 10.1128/CVI.00060-07
URI: http://repositorio.bc.ufg.br/handle/ri/15555
Data de publicação: Set-2007
Aparece nas coleções:IPTSP - Artigos publicados em periódicos

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