Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/15556
Tipo do documento: Artigo
Título: Ação anti-inflamatória do amitraz pela inibição da produção de interferon gama e da atividade mitocondrial de linfócitos T.
Título(s) alternativo(s): Anti- inflammatory effect of amitraz due to inhibition of the gamma- interferon production and mitochondrial activity of the T lymphocytes
Acción anti-inflamatoria del amitraz por la inhibición de la producción de interferon gama y de la actividad mitocondral de los linfocitos T.
Autor: Di Filippo, Paula Alessandra
Sousa, Raimundo Vicente de
Vieira, Leda Quercia
Santana, Gilcinéa de Cássia
Abrahamsohn, Ises de Almeida
Oliviera, Milton Adriano Pelli de
Resumo: Amitraz, a formamidine pesticide, is one of the selected drugs for the treatment of the canine demodectic mange. Inflammatory alterations are found associated with the presence of high numbers of the mite Demodex canis in the skin. The reasons for the indication of amitraz are based only on its acaricide action. Some authors have suggested analgesic and anti-inflammatory properties for this drug, but few studies have been done to understand the mechanisms that prove such effect. To validate this suggested action, splenic cells from C3H/He mice were cultured and stimulated with either 5μg/mL of Concanavalin A (Con A) or 10μg/mL of lipopolysaccharide (LPS) from Escherichia coli 0111:B4 in the presence of 0.00; 0.78; 1.56; 3.13; 6.25 and 12.50μg/mL of amitraz diluted in arol or equivalent amount of solvent. The viability of the cells was significantly reduced when these cells were cultured for 48 hours with amounts equal or higher than 3.13 μg/mL of amitraz. The mitochondrial activity of spleen cells, analyzed by the MTT reduction method, did not change when amitraz was used in concentrations equal or higher than 1.56 μg/mL, although this activity was significantly reduced when the spleen cells were stimulated with Con A and then treated with amitraz. The proliferation of T lymphocytes stimulated with Con A did not change when amitraz was used in the concentrations equal or higher than 1.56μg/mL. The proliferation of B lymphocytes also did not change when spleen cells were treated with LPS. The production of IFN-γ was inhibited by amitraz in concentrations equal or higher than 1.56μg/mL. The results described here demonstrate that one of the possible mechanisms for the anti-inflammatory effect of amitraz relates to its ability of inhibiting the T lymphocyte metabolism and IFN-γ production, without affecting the proliferation of these cells.
Abstract: El amitraz es un pesticida formamidínico y una de las drogas escogidas en el tratamiento de la sarna demodésica canina. Alteraciones inflamatorias se encuentran asociadas con la presencia, en número elevado, del ácaro Demodex canis en la piel. Las justificativas para la indicación del amitraz se basan apenas en su acción acaricida. Aunque algunos autores han sugerido una posible acción analgésica y antiinflamatoria, poco se ha hecho para entender los mecanismos que proporcionan tal efecto. Para verificar esta supuesta acción, células esplénicas de ratones C3H/He, fueron cultivadas y estimuladas con 5 μg/ml de Concanavalina A (Con A) o 10 μg/ml de lipopolisacáridos (LPS) de Escherichia coli 0111:B4, en presencia de 0; 0.78; 1.56; 3.13; 6.25 y 12.50 μg/ml de amitraz diluido en arol, o una cantidad equivalente de diluyente. La viabilidad de las células fue significativamente reducida cuando cultivadas por 48 horas en cantidades iguales o superiores a 3,13 μg/ml de amitraz. La actividad mitocondrial de los esplenocitos, medida por medio de la reducción del MTT, no se alteró cuando estos fueron tratados con amitraz en concentraciones iguales a 1,56 μg/ml. Por lo tanto, esta actividad fue significativamente reducida cuando los esplenocitos fueron estimulados con Con A y posteriormente tratados con amitraz. La proliferación de los linfocitos T estimulada por Con A no se alteró cuando se utilizó el amitraz en una concentración (1,56 μg/ml) capaz de inhibir el metabolismo de estas células. La proliferación de los linfocitos B tampoco mostró ser alterada cuando los esplenocitos fueron tratados con LPS. La producción de IFN-γ fue inhibida por el amitraz en concentraciones iguales o superiores a 1,56 μg/ml. Los resultados presentados muestran que uno de los posibles mecanismos de la acción antiinflamatoria del amitraz se debe a su capacidad de inhibir el metabolismo y la producción de IFN-γ por los linfocitos T, pero sin afectar su proliferación.
Amitraz, a formamidine pesticide, is one of the selected drugs for the treatment of the canine demodectic mange. Inflammatory alterations are found associated with the presence of high numbers of the mite Demodex canis in the skin. The reasons for the indication of amitraz are based only on its acaricide action. Some authors have suggested analgesic and anti-inflammatory properties for this drug, but few studies have been done to understand the mechanisms that prove such effect. To validate this suggested action, splenic cells from C3H/He mice were cultured and stimulated with either 5μg/mL of Concanavalin A (Con A) or 10μg/mL of lipopolysaccharide (LPS) from Escherichia coli 0111:B4 in the presence of 0.00; 0.78; 1.56; 3.13; 6.25 and 12.50μg/mL of amitraz diluted in arol or equivalent amount of solvent. The viability of the cells was significantly reduced when these cells were cultured for 48 hours with amounts equal or higher than 3.13 μg/mL of amitraz. The mitochondrial activity of spleen cells, analyzed by the MTT reduction method, did not change when amitraz was used in concentrations equal or higher than 1.56 μg/mL, although this activity was significantly reduced when the spleen cells were stimulated with Con A and then treated with amitraz. The proliferation of T lymphocytes stimulated with Con A did not change when amitraz was used in the concentrations equal or higher than 1.56μg/mL. The proliferation of B lymphocytes also did not change when spleen cells were treated with LPS. The production of IFN-γ was inhibited by amitraz in concentrations equal or higher than 1.56μg/mL. The results described here demonstrate that one of the possible mechanisms for the anti-inflammatory effect of amitraz relates to its ability of inhibiting the T lymphocyte metabolism and IFN-γ production, without affecting the proliferation of these cells.
Palavras-chave: Amitraz
Sarna demodécica
Linfócito T.
Interferon gama
Arol
Amitraz
Linfocitos T.
Sarna demodésica
Interferón gama
Arol
Amitraz
Canine Demodex
T lymphocytes
IFN-γ
Arol
País: Brasil
Unidade acadêmica: Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
Citação: DI FILLIPO, P. A.; SOUSA, R. V.; VIEIRA, L. Q.; SANTANA, G. C.; ABRAHAMSOHN, I. A.; OLIVEIRA, M. A. P. Ação anti-inflamatória do amitraz pela inibição da produção de interferon gama e da atividade mitocondrial de linfócitos T. ARS Veterinária, Jaboticabal, v. 22, n. 2, p. 138-145, 2006.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.15361/2175-0106.2006v22n2p138-145
Identificador do documento: 10.15361/2175-0106.2006v22n2p138-145
URI: http://repositorio.bc.ufg.br/handle/ri/15556
Data de publicação: 2006
Aparece nas coleções:IPTSP - Artigos publicados em periódicos

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