Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/15614
Tipo do documento: Artigo
Título: Mycobacterium tuberculosis epitope-specific interferon-g production in healthy Brazilians reactive and non-reactive to tuberculin skin test
Autor: Silva, Bosco Christiano Maciel da
Grassi, Maria Fernanda Rios
Coutinho Junior, Raimundo
Mascarenhas, Rita Elizabeth Moreira
Olavarria Gallazzi, Viviana Nilla
Borgo, Adriana Coutinho
Kalil Filho, Jorge Elias
Cunha Neto, Edecio
Fonseca, Simone Gonçalves da
Abstract: The interferon (IFN)-γ response to peptides can be a useful diagnostic marker of Mycobacterium tuberculosis (MTB) latent infection. We identified promiscuous and potentially protective CD4 + T-cell epitopes from the most conserved regions of MTB antigenic proteins by scanning the MTB antigenic proteins GroEL2, phosphate-binding protein 1 precursor and 19 kDa antigen with the TEPITOPE algorithm. Seven peptide sequences predicted to bind to multiple human leukocyte antigen (HLA)-DR molecules were synthesised and tested with IFN-γ enzyme-linked immunospot (ELISPOT) assays using peripheral blood mononuclear cells (PBMCs) from 16 Mantoux tuberculin skin test (TST)-positive and 16 TST-negative healthy donors. Eighty-eight percent of TST-positive donors responded to at least one of the peptides, compared to 25% of TST-negative donors. Each individual peptide induced IFN-γ production by PBMCs from at least 31% of the TST-positive donors. The magnitude of the response against all pep- tides was 182 ± 230 x 10 6 IFN-γ spot forming cells (SFC) among TST-positive donors and 36 ± 62 x 10 6 SFC among TST-negative donors (p = 0.007). The response to GroEL2 (463-477) was only observed in the TST-positive group. This combination of novel MTB CD4 T-cell epitopes should be tested in a larger cohort of individuals with latent tuberculosis (TB) to evaluate its potential to diagnose latent TB and it may be included in ELISPOT-based IFN-γ assays to identify individuals with this condition.
Palavras-chave: Mycobacterium tuberculosis
Mantoux tuberculin skin test
Epitopes
IFN-γ ELISPOT
Latent tuberculosis
País: Brasil
Unidade acadêmica: Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
Citação: SILVA, Bosco Christiano Maciel da; GRASSI, Maria Fernanda Rios; COUTINHO, Raimundo; MASCARENHAS, Rita Elizabeth Moreira; OLAVARRIA, Viviana Nilla; COUTINHO-BORGO, Adriana; KALIL, Jorge; CUNHA-NETO, Edecio; FONSECA, Simone Gonçalves. Mycobacterium tuberculosis epitope-specific interferon-g production in healthy Brazilians reactive and non-reactive to tuberculin skin test. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 109, n. 8, p. 999-1004, Dec. 2014.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1590/0074-0276140193
Identificador do documento: 10.1590/0074-0276140193
URI: http://repositorio.bc.ufg.br/handle/ri/15614
Data de publicação: Dez-2014
Aparece nas coleções:IPTSP - Artigos publicados em periódicos

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