1H HRMAS NMR spectroscopy and chemometrics for evaluation of metabolic changes in citrus sinensis caused by xanthomonas axonopodis pv. citri
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Data
2012-05
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Resumo
Xanthomonas axonopodis (Xac) bacterium causes one of the most feared and untreatable
diseases in citriculture: citrus canker. To understand the response mechanisms of orange trees
when attacked by Xac, leaves and fruits of Citrus sinensis were directly evaluated by HRMAS
NMR (high resolution magic angle spinning nuclear magnetic resonance) spectroscopy. This
technique allows the analysis of samples without laborious pre-treatments and also allows access
to important information about chemical composition of samples. The orange tree leaves and
fruit peels investigated in this study demonstrated the biochemical changes caused by Xac. Aided
by chemometric analysis, the HRMAS NMR results show relevant changes in amino acids,
carbohydrates, organic acids and terpenoids content.
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1H HRMAS NMR, Citrus canker, Citrus, Xanthomonas axonopodis, Metabolic fingerprint, Chemometrics
Citação
SILVA, Lorena .M. A. et al. 1H HRMAS NMR spectroscopy and chemometrics for evaluation of metabolic changes in citrus sinensis Caused by Xanthomonas axonopodis pv. citri. Journal of the Brazilian Chemical Society, Campinas, v. 23, n. 6, p. 1054-1061, May 2012.