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Tipo do documento: Artigo
Título: Trichoderma reesei mycoparasitism against Pythium ultimum is coordinated by G-alpha protein GNA1 signaling
Autor: Monteiro, Valdirene Neves
Steindorff, Andrei Stecca
Almeida, Fausto Bruno dos Reis
Lopes, Fabyano Alvares Cardoso
Ulhoa, Cirano José
Félix, Carlos Roberto
Silva, Roberto Nascimento
Abstract: Trichoderma reesei (Hypocrea jecorina) is widely explored in industry and its potential for using in agriculture as a biocontrol agent against phytophatogenic fungi has just began to be investigated. We have investigated the involvement of G proteins during mycoparasitism against plant pathogens. Here we described the role of GNA1, a G-alpha protein that belongs to α i group in Cell Wall Degrading Enzymes (CWDEs) production by T. reesei during antagonism against Pythium ultimum. For that, two mutants were used: Δgna1 and gna1QL (=constitutively activated version of GNA1). The gna1QL mutant of T. reesei, like the parental TU-6, inhibited the growth of P. ultimum in plate confrontation assay and grew faster than the parental TU-6 while the Δgna1 did not grow over P. ultimum. Scanning electron microscopy showed that the gna1QL mutant promoted more morphological alterations of P. ultimum cell wall than the parental TU-6 while the Δgna1 caused no effects. The mutant Δgna1 showed less CWDEs activity than gna1QL and TU-6 during in vitro cultivations. The gna1QL mutant showed a better performance in production of CWDEs such as endochitinase, N-Acetyl-β-D-glucosaminidase (NAGase), lipase and acid phosphatase, after 72 hours of incubation. However, the parental TU-6 showed higher cellulase activity than gna1QL and Δgna1. The intracellular content of cAMP in the strains after 72 hours of incubation in the presence of P. ultimum cell wall was: gna1QL (79.85 ± 12), Δgna1 (268.65 ± 8.5) and TU-6 (109.70 ± 9.2) pmol/mg protein. RT-qPCR results showed a low level of transcripts of mycoparasitism-specific genes in Δgna1 strain. We therefore suggest that the production of some CWDEs during mycoparasitism by T. reesei against P. ultimum can be mediated by GNA1 activity or cAMP levels.
Palavras-chave: Degrading enzymes
Trichoderma reesei
País: Outros
Unidade acadêmica: Instituto de Ciências Biológicas - ICB (RG)
Citação: MONTEIRO, Valdirene Neves; STEINDORF, Andrei Stecca; ALMEIDA, Fausto Bruno dos Reis; LOPES, Fabyano Alvares Cardoso; ULHOA, Cirano José; FELIX, Carlos Roberto; SILVA, Roberto Nascimento. Trichoderma reesei mycoparasitism against Pythium ultimum is coordinated by G-alpha protein GNA1 signaling. Journal of Microbial & Biochemical Technology, Traian, v. 7, n. 1, p. 1-7, 2015.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.4172/1948-5948.1000173
Identificador do documento: 10.4172/1948-5948.1000173
Data de publicação: 2014
Aparece nas coleções:ICB - Artigos publicados em periódicos

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