Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/15730
Tipo do documento: Artigo
Título: Identification of differentially expressed genes from Trichoderma harzianum during growth on cell wall of Fusarium solani as a tool for biotechnological application
Autor: Vieira, Pabline Marinho
Coelho, Alexandre Siqueira Guedes
Steindorff, Andrei Stecca
Siqueira, Saulo José Linhares de
Silva, Roberto do Nascimento
Ulhoa, Cirano José
Abstract: Background: The species of T. harzianum are well known for their biocontrol activity against many plant pathogens. However, there is a lack of studies concerning its use as a biological control agent against F. solani, a pathogen involved in several crop diseases. In this study, we have used subtractive library hybridization (SSH) and quantitative real-time PCR (RT-qPCR) techniques in order to explore changes in T. harzianum genes expression during growth on cell wall of F. solani (FSCW) or glucose. RT-qPCR was also used to examine the regulation of 18 genes, potentially involved in biocontrol, during confrontation between T. harzianum and F. solani. Results: Data obtained from two subtractive libraries were compared after annotation using the Blast2GO suite. A total of 417 and 78 readable EST sequence were annotated in the FSCW and glucose libraries, respectively. Functional annotation of these genes identified diverse biological processes and molecular functions required during T. harzianum growth on FSCW or glucose. We identified various genes of biotechnological value encoding to proteins which function such as transporters, hydrolytic activity, adherence, appressorium development and pathogenesis. Fifteen genes were up-regulated and sixteen were down-regulated at least at one-time point during growth of T. harzianum in FSCW. During the confrontation assay most of the genes were up-regulated, mainly after contact, when the interaction has been established. Conclusions: This study demonstrates that T. harzianum expressed different genes when grown on FSCW compared to glucose. It provides insights into the mechanisms of gene expression involved in mycoparasitism of T. harzianum against F. solani. The identification and evaluation of these genes may contribute to the development of an efficient biological control agent.
Palavras-chave: T. harzianum
Mycoparasitism
F. solani
Gene expression
Subtractive library hybridization
País: Gra-bretanha
Unidade acadêmica: Instituto de Ciências Biológicas - ICB (RG)
Citação: VIEIRA, Pabline Marinho; COELHO, Alexandre Siqueira Guedes; STEINDORFF, Andrei Stecca; SIQUEIRA, Saulo José Linhares de; SILVA, Roberto do Nascimento; ULHOA, Cirano José. Identification of differentially expressed genes from Trichoderma harzianum during growth on cell wall of Fusarium solani as a tool for biotechnological application. BMC Genomics, London, v. 14, n. 177, p 1-11, 2013.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1186/1471-2164-14-177
Identificador do documento: 10.1186/1471-2164-14-177
URI: http://repositorio.bc.ufg.br/handle/ri/15730
Data de publicação: 2013
Aparece nas coleções:ICB - Artigos publicados em periódicos

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