Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/15732
Tipo do documento: Artigo
Título: Biochemical and structural characterization of Amy1: an alpha-amylase from Cryptococcus flavus expressed in Saccharomyces cerevisiae
Autor: Galdino, Alexsandro Sobreira
Silva, Roberto do Nascimento
Lottermann, Muriele Taborda
Álvares, Alice Cunha Morales
Moraes, Lidia Maria Pepe de
Torres, Fernando Araripe Gonçalves
Freitas, Sonia Maria de
Ulhoa, Cirano José
Abstract: An extracellular alpha-amylase (Amy1) whose gene from Cryptococcus flavus was previously expressed in Saccharomyces cerevisiae was purified to homogeneity (67 kDa) by ion-exchange and molecular exclusion chromatography. The enzyme was activated by NH 4 + and inhibited by Cu +2 and Hg +2 . Significant biochemical and structural discrepancies between wild-type and recombinant α- amylase with respect to K m values, enzyme specificity, and secondary structure content were found. Far-UV CD spectra analysis at pH 7.0 revealed the high thermal stability of both proteins and the difference in folding pattern of Amy1 compared with wild-type amylase from C. flavus, which reflected in decrease (10-fold) of enzymatic activity of recombinant protein. Despite the differences, the highest activity of Amy1 towards soluble starch, amylopectin, and amylase, in contrast with the lowest activity of Amy1 w , points to this protein as being of paramount biotechnological importance with many applications ranging from food industry to the production of biofuels.
País: Gra-bretanha
Unidade acadêmica: Instituto de Ciências Biológicas - ICB (RG)
Citação: GALDINO, Alexsandro Sobreira; SILVA, Roberto Nascimento; LOTTERMANN, Muriele Taborda; ALVARES, Alice Cunha Morales; MORAES, Lídia Maria Pepe de; TORRES, Fernando Araripe Gonçalves; FREITAS, Sonia Maria de; ULHOA, Cirano Jose. Biochemical and structural characterization of Amy1, an alpha-amylase from Cryptococcus flavus expressed in Saccharomyces cerevisiae. Enzyme Research, London, v. 2011, p. 1-7, 2011.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.4061/2011/157294
Identificador do documento: 10.4061/2011/157294
URI: http://repositorio.bc.ufg.br/handle/ri/15732
Data de publicação: 2011
Aparece nas coleções:ICB - Artigos publicados em periódicos

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