The ruthenium complex cis-(dichloro)tetrammineruthenium(III) chloride induces apoptosis and damages DNA in murine sarcoma 180 cells
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2010-09
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Resumo
Ruthenium(III) complexes are increasingly attracting the interest of researchers due to their promising pharmacological
properties. Recently, we reported that the cis-(dichloro)tetrammineruthenium(III) chloride compound has cytotoxic
effects on murine sarcoma 180 (S-180) cells. In an effort to understand the mechanism responsible for their
cytotoxicity, study we investigated the genotoxicity, cell cycle distribution and induction of apoptosis caused by cis-
(dichloro)tetrammineruthenium(III) chloride in S-180 tumour cells. cis-(dichloro)tetrammineruthenium(III) chloride
treatment induced signifi cant DNA damage in S-180 cells, as detected by the alkaline comet assay. In the cell cycle
analysis, cis-(dichloro)tetrammineruthenium(III) chloride caused an increase in the number of cells in G1 phase,
accompanied by a decrease in the S and G2 phases after 24 h of treatment. In contrast, the cell cycle distribution of
S-180 cells treated with cis-(dichloro)tetrammineruthenium(III) chloride for 48 h showed a concentration-dependent
increase in the sub-G1 phase (indicating apoptosis), with a corresponding decrease in cells in the G1, S and G2 phases.
In addition, cis-(dichloro)tetrammineruthenium(III) chloride treatment induced apoptosis in a time-dependent manner,
as observed by the increased numbers of annexin V-positive cells. Taken together, these fi ndings strongly demonstrate
that DNA damage, cell cycle changes and apoptosis may correlate with the cytotoxic effects of cis-(dichloro)tetramm-
ineruthenium(III) chloride on S-180 cells.
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Apoptosis, S-180, Cis-(dichloro)tetrammineruthenium(III) chloride, Cell cycle, DNA damage
Citação
LIMA, Aliny Pereira de; PEREIRA, Flavia de Castro; VILANOVA-COSTA, Cesar Augusto Sam Tiago; RIBEIRO, Alessandra de Santana Braga Barbosa; PAVANIN, Luiz Alfredo; SANTOS, Wagner Batista dos; SILVEIRA-LACERDA, Elisângela de Paula. The ruthenium complex cis-(dichloro)tetrammineruthenium(III) chloride induces apoptosis and damages DNA in murine sarcoma 180 (S-180) cells. Journal of Biosciences, Berlim, v. 35, n. 3, p. 371-378, Sept. 2010.