Utilização do cultivo in vitro de folículos pré-antrais suínos inclusos em tecido ovariano (in situ) como modelo de avaliação da eficiência de medicamentos homeopáticos na foliculogênese inicial
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Data
2018
Título da Revista
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Editor
José Henrique Stringhini
Resumo
This study investigated the effect of homeopathic medicine (Pulsatilla nigricans and homeopathic
follicle stimulating hormone – FSH) and the complex (Bos Stress Fertilis) on the initial
folliculogenesis, using the in vitro culture of preantral follicles as in vitro model. For this, swine ovarian fragments were cultured for one or seven days in α-MEM+ in the absence (cultured control)
or presence of homeopathic FSH (6 cH), Pulsatilla (6 CH), Bos Stress Fertilis (6 CH), grain alcohol
(50% v / v) or recombinant FSH (50 ng / ml) added daily. Uncultured ovarian fragments (fresh
control) or cultured for one and seven days were processed for classical histology. Only the
homeopathic complex Bos Stress Fertilis maintained the percentage of follicular survival after seven
days of culture in relation to the uncultured control and α-MEM+. Regarding follicular growth, only
the addition of homeopathic FSH increased the follicular diameter when compared to the uncultured
control and a-MEM+. Thus, it can be concluded that the addition of the homeopathic remedies Bos
Stress fertilis and homeopathic FSH (6 CH) improved, respectively, survival and in vitro growth of
swine preantral follicles included in ovarian tissue.
Descrição
Palavras-chave
Folículo pré-antral, FSH, Homeopatia, Ovário, Suíno, Homeopathy, Ovary, Preantral follicle, Swine
Citação
NUNES, Romão; LIMA, Laritza Ferreira de; ROCHA, Rebeca Magalhães Pedrosa; OLIVEIRA, Letícia; CAMPELLO, Claudio Cabral; FIGUEIREDO, Jose Ricardo. Utilização do cultivo in vitro de folículos pré-antrais suínos inclusos em tecido ovariano (in situ) como modelo de avaliação da eficiência de medicamentos homeopáticos na foliculogênese inicial. Ciência Animal Brasileira, Goiânia, v. 19, e48658, 2018. Disponível em: <https://www.revistas.ufg.br/vet/article/view/e-48658/25968>.