Characterization of rue extract and its potential for controlling rice blast
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Data
2015-12
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Resumo
The objective of this work was to purify and standardize the rue (Ruta graveolens) extract and
evaluate its effect on Magnaporthe oryzae as an alternative to the integrated management of rice blast. The
drug was characterized, the liquid extract was obtained, and the methodology for quantifying the standard
markers psoralen and bergapten was validated. Rue extract and the markers, solely or in combination, were
assayed in vitro, as well as in greenhouse conditions, for their ability to suppress leaf blast, by the evaluation of
mycelial growth, conidial germination, and appressorium formation. Rue extract inhibited M. oryzae mycelial
growth (100%), conidial germination (LD 50 =0.237 mg), and the appressorium formation (LD 50 =0.121 mg);
besides, the extract reduced leaf blast severity by 80.84%. Fluorescence microscopy showed that rue extract
did not damage M. oryzae cell wall and plasma membrane, indicating another mode of action. Rue extract has
a great potential for controlling rice leaf blast.
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Magnaporthe oryzae, Psoralen, Ruta graveolens, Bergapten, Magnaporthe oryzae, Psoraleno, Ruta graveolens, Bergapteno
Citação
REIS, Karinna Bannach et al. Characterization of rue extract and its potential for controlling rice blast. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 50, n. 12, p. 1121-1130, Dec. 2015.