Composição química do óleo essencial e avaliação da atividade antimicrobiana do óleo essencial, extrato etanólico bruto e frações das folhas de Spiranthera odoratissima A. St.-Hil.
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Data
2013
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Resumo
Spiranthera odoratissima (“manacá”) has been popularly used as a blood
cleanser, for liver and kidney diseases (tea from the leaves), as well as for muscle and stomach
pains, headache and liver disorders (tea from the roots). The aim of this study was to evaluate
the chemical composition of the essential oil and the antimicrobial activity of essential oil, crude
ethanol extract and fractions of S. odoratissima leaves against Gram-positive and negative
bacteria and Candida albicans. The crude extract of the leaves was obtained by maceration
and was concentrated in a rotavapor, while the fractions were obtained by partition on column
filter. The powdered leaves underwent hydrodistillation in a Clevenger apparatus and the
obtained essential oil was analyzed by GC/MS. The antimicrobial activity was evaluated by
using the agar dilution method for determining the minimum inhibitory concentration (MIC). The
major constituents of the essential oil were β-caryophyllene (20.64%), γ-muurolene (17.70%),
bicyclogermacrene (14.73%) and δ-cadinene (13.40%). The main results for S. odoratissima
antimicrobial activity were found against Staphylococcus epidermidis (crude ethanol extract,
MIC of 0.098 mg/mL), C. albicans (hexane fraction, MIC of 0.049 mg/mL), Bacillus cereus
(dichloromethane fraction, MIC of 0.098 mg/mL), Micrococcus roseus (ethyl acetate fraction,
MIC of 0.049 mg/mL) and M. roseus, Micrococcus luteus, B. cereus and C. albicans (methanol
fraction, MIC of 0.391 mg/mL).
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Plantas medicinais, β-cariofileno, Manacá, Cerrado, Rutaceae, Medicinal plants, Rutaceae, “Manacá”, Cerrado, β-caryophyllene
Citação
CHAIBUB, B. A. et al. Composição química do óleo essencial e avaliação da atividade antimicrobiana do óleo essencial, extrato etanólico bruto e frações das folhas de Spiranthera odoratissima A. St.-Hil. Revista Brasileira de Plantas Medicinais, Campinas, v. 15, n. 2, p. 225-229, 2013.