Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/16098
Tipo do documento: Artigo
Título: Improvement of starch digestion using a-amylase entrapped in pectin? Polyvinyl alcohol blend
Autor: Cruz, Maurício Vicente
Fernandes, Kátia Flávia
Cysneiros, Cristine dos Santos Settimi
Ferreira, Reginaldo Nassar
Caramori, Samantha Salomão
Abstract: Polyvinyl alcohol (PVA) and pectin blends were used to entrap α-amylase (Termamyl) using glutaraldehyde as a cross-linker. The effect of glutaraldehyde concentration (0.25, 0.5, 0.75, 1.0, and 1.25%) on the activity of the immobilized enzyme and rate of enzyme released was tested during a 24 h period. Characteristics of the material, such as scanning electron microscopy (SEM), tensile strength (TS), elongation, and rate of dissolution in water (pH 5.7), ruminal buffering solution (pH 7.0), and reactor containing 0.1 mol L −1 sodium phosphate buffer (pH 6.5), were also analyzed. SEM results showed that the surfaces of the pectin/PVA/amylase films were highly irregular and rough. TS values increased as a function of glutaraldehyde concentration, whereas percentage of elongation (%E) decreased. Pectin/PVA/amylase films presented similar values of solubility in the tested solvents. The material obtained with 0.25% glutaraldehyde performed best with repeated use (active for 24 h), in a phosphate buffer reactor. By contrast, the material obtained with 1.25% glutaraldehyde presented higher performance during in vitro testing using an artificial rumen. The results suggest that pectin/PVA/amylase is a highly promising material for biotechnological applications.
País: Gra-bretanha
Unidade acadêmica: Instituto de Ciências Biológicas - ICB (RG)
Citação: CRUZ, Maurício et al. Improvement of starch digestion using a-amylase entrapped in pectin? Polyvinyl alcohol blend. Biomed Research International, London, v. 2015, p. 1-9, 2015.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1155/2015/145903
Identificador do documento: 10.1155/2015/145903
URI: http://repositorio.bc.ufg.br/handle/ri/16098
Data de publicação: 2015
Aparece nas coleções:ICB - Artigos publicados em periódicos

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