Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/16112
Tipo do documento: Artigo
Título: Optimal conditions for continuous immobilization of Pseudozyma hubeiensis (strain HB85A) lipase by adsorption in a packed-bed reactor by response surface methodology
Autor: Rodrigues, Roberta da Silva Bussamara
Dall' Agnol, Luciane
Schrank, Augusto
Fernandes, Kátia Flávia
Vainstein, Marilene Henning
Abstract: This study aimed to develop an optimal continuous process for lipase immobilization in a bed reactor in order to investigate the possibility of large-scale production. An extracellular lipase of Pseudozyma hubeiensis (strain HB85A) was immobilized by adsorption onto a polystyrene-divinylbenzene support. Furthermore, response surface methodology (RSM) was employed to optimize enzyme immobilization and evaluate the optimum temperature and pH for free and immobilized enzyme. The optimal immobilization conditions observed were 150 min incubation time, pH 4.76, and an enzyme/support ratio of 1282 U/g support. Optimal activity temperature for free and immobilized enzyme was found to be 68 ◦ C and 52 ◦ C, respectively. Optimal activity pH for free and immobilized lipase was pH 4.6 and 6.0, respectively. Lipase immobilization resulted in improved enzyme stability in the presence of nonionic detergents, at high temperatures, at acidic and neutral pH, and at high concentrations of organic solvents such as 2-propanol, methanol, and acetone.
País: Gra-bretanha
Unidade acadêmica: Instituto de Ciências Biológicas - ICB (RG)
Citação: BUSSAMARA, Roberta et al. Optimal conditions for continuous immobilization of Pseudozyma hubeiensis (strain HB85A) lipase by adsorption in a packed-bed reactor by response surface methodology. Enzyme Reseach, London, v. 2012, p. 1-12, 2012.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1155/2012/329178
Identificador do documento: 10.1155/2012/329178
URI: http://repositorio.bc.ufg.br/handle/ri/16112
Data de publicação: 2012
Aparece nas coleções:ICB - Artigos publicados em periódicos

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