Immobilized horseradish peroxidase on discs of polyvinyl alcohol-glutaraldehyde coated with polyaniline
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2012
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Discs of network polyvinyl alcohol-glutaraldehyde (PVAG) were synthesized and coated with polyaniline (PANI) using
glutaraldehyde as a chemical arm (PVAG-PANIG-HRP disc). The best conditions for the immobilization were established as
about 1.0 mg mL − 1 of protein, for 60 min and pH 5.5. The soluble enzyme lost all of its activity after incubation at 70 ◦ C for
15 min, whereas the PVAG-PANIG-HRP disc retained about half of the initial activity for pyrogallol. The same PVAG-PANIG-
HRP disc was used consecutively three times without any activity lossbut presented 25% of the initial activity after the 7th use.
PVAG-PANIG-HRP disc retained approximately 80% and 60% of its initial activity after 60 and 80 days of storage, respectively.
Resorcinol, m-cresol, catechol, pyrogallol, α-naphthol, βnaphthol, and 4, 4 -diaminodiphenyl benzidine were efficiently oxidized
by the PVAG-PANIG-HRP disc (from about 70% to 90%), and it was less efficient towards aniline, phenol, and 2-nitrosonaphthol.
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CARAMORI, Samantha Salomão; FERNANDES, Kátia Flávia; CARVALHO JUNIOR, Luiz Bezerra de. Immobilized horseradish peroxidase on discs of polyvinyl alcohol-glutaraldehyde coated with polyaniline. The Scientific World Journal, London, v. 2012, p. 1-8, 2012.