Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/16114
Tipo do documento: Artigo
Título: Immobilized horseradish peroxidase on discs of polyvinyl alcohol-glutaraldehyde coated with polyaniline
Autor: Caramori, Samantha Salomão
Fernandes, Kátia Flávia
Carvalho Junior, Luiz Bezerra de
Abstract: Discs of network polyvinyl alcohol-glutaraldehyde (PVAG) were synthesized and coated with polyaniline (PANI) using glutaraldehyde as a chemical arm (PVAG-PANIG-HRP disc). The best conditions for the immobilization were established as about 1.0 mg mL − 1 of protein, for 60 min and pH 5.5. The soluble enzyme lost all of its activity after incubation at 70 ◦ C for 15 min, whereas the PVAG-PANIG-HRP disc retained about half of the initial activity for pyrogallol. The same PVAG-PANIG- HRP disc was used consecutively three times without any activity lossbut presented 25% of the initial activity after the 7th use. PVAG-PANIG-HRP disc retained approximately 80% and 60% of its initial activity after 60 and 80 days of storage, respectively. Resorcinol, m-cresol, catechol, pyrogallol, α-naphthol, βnaphthol, and 4, 4 -diaminodiphenyl benzidine were efficiently oxidized by the PVAG-PANIG-HRP disc (from about 70% to 90%), and it was less efficient towards aniline, phenol, and 2-nitrosonaphthol.
País: Gra-bretanha
Unidade acadêmica: Instituto de Ciências Biológicas - ICB (RG)
Citação: CARAMORI, Samantha Salomão; FERNANDES, Kátia Flávia; CARVALHO JUNIOR, Luiz Bezerra de. Immobilized horseradish peroxidase on discs of polyvinyl alcohol-glutaraldehyde coated with polyaniline. The Scientific World Journal, London, v. 2012, p. 1-8, 2012.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1100/2012/129706
Identificador do documento: 10.1100/2012/129706
URI: http://repositorio.bc.ufg.br/handle/ri/16114
Data de publicação: 2012
Aparece nas coleções:ICB - Artigos publicados em periódicos

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
Artigo - Samantha Salomão Caramori - 2012.pdf1,92 MBAdobe PDFThumbnail
Baixar/Abrir


Este item está licenciada sob uma Licença Creative Commons Creative Commons