Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/16218
Tipo do documento: Artigo
Título: Selection of optimized candidate reference genes for qRT-PCR normalization in rice (Oryza sativa L.) during Magnaporthe oryzae infection and drought
Autor: Bevitori, Rosangela
Oliveira, Marília Barros
Sa, Maria Fatima Grossi de
Lanna, Anna Cristina
Silveira, R. D. da
Silva, Silvana Petrofeza da
Resumo: Drought and rice blast disease caused by Magnaporthe oryzae are two of the most serious threats to global rice production. To explore the mechanisms underlying gene expression induced in rice by stresses, studies involving transcriptome analyses have been conducted over the past few years. Thus, it is crucial to have a reliable set of reference genes to normalize the expression levels of rice genes affected by different stresses. To identify potential reference genes for studies of the differential expression of target genes in rice under M. oryzae infection and drought conditions, the present study evaluated five housekeeping genes for the normalization of gene expression. The stability of the expression of these genes was assessed using the analytical software packages geNorm and NormFinder. For all samples analyzed, the stability rank was UBQ5 > GAPDH > eIF-4α > β-TUB > 18S rRNA. The data showed that the UBQ5, GAPDH, and eIF-4α genes are appropriate, high-performing reference genes and will be highly useful in future expression studies of fungal infections and drought in rice.
Palavras-chave: Housekeeping genes
Normalization
Gene expression
Real-time quantitative reverse transcription polymerase chain reaction
Stable expression
País: Brasil
Unidade acadêmica: Instituto de Ciências Biológicas - ICB (RG)
Citação: BEVITORI, R. et al. Selection of optimized candidate reference genes for qRT-PCR normalization in rice (Oryza sativa L.) during Magnaporthe oryzae infection and drought. Genetics and Molecular Research, Ribeirão Preto, v. 13, n. 4, p. 9795-9805, 2014.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.4238/2014.November.27.7
Identificador do documento: 10.4238/2014.November.27.7
URI: http://repositorio.bc.ufg.br/handle/ri/16218
Data de publicação: 2014
Aparece nas coleções:ICB - Artigos publicados em periódicos

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