Intraspecific differentiation of Hancornia speciosa revealed by simple sequence repeat and random amplified polymorphic DNA markers
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2015-12
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Resumo
Hancornia speciosa, popularly known as mangabeira, is a fruit tree native to the Brazilian Cerrado that shows great economic potential, due to its multiple uses. Intraspecific classification of this species is difficult because it shows high morphological diversity. An early study of the species reported that there are six botanic varieties that differ morphologically mainly in the shapes of their leaves and flowers. Except to note the wide morphological variation and economic potential of this species, few studies have been published about the genetic diversity of mangabeira. Knowledge of the genetic variability of this species among
populations would be useful for genetic conservation and breeding
programs. Therefore, we tested the transferability of 12 simple sequence
repeats from expressed sequence tags (EST-SSRs) from Catharanthus
roseus to H. speciosa and used 10 random amplified polymorphic DNA
markers to evaluate the genetic variability among botanical varieties of
H. speciosa. We obtained a high transferability frequency of EST-SSR
markers from C. roseus to H. speciosa (75%). However, EST-SSR markers
showed low heterozygosity and locus variability (two or three alleles by
locus), which suggest low genetic diversity in the mangabeira samples. The
Jaccard dissimilarity index and an examination of geographic distances
indicated a non-spatial structuring of the genetic variability. Our markers
were unable to distinguish H. speciosa botanical varieties.
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Palavras-chave
Cerrado, Genetic variability, Hancornia speciosa, Mangabeira, Molecular markers, Transferability frequency
Citação
NOGUEIRA, C. A. et al. Intraspecific differentiation of Hancornia speciosa revealed by simple sequence repeat and random amplified polymorphic DNA markers. Genetics and Molecular Research, Ribeirão Preto, v. 14, n. 4, p. 15996-16005, Dec. 2015.