Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/16229
Tipo do documento: Artigo
Título: Effect of different carbon sources on proteases secreted by the fungal pathogen Sclerotinia sclerotiorum during Phaseolus vulgaris infection
Autor: Bueno, E. A.
Oliveira, Marília Barros
Andrade, Rosangela Vieira de
Lobo Junior, Murillo 
Silva, Silvana Petrofeza da
Abstract: Sclerotinia sclerotiorum (Sclerotiniaceae) is a plant pathogenic fungus that causes white mold disease in vegetable crops, including the common bean (Phaseolus vulgaris). Proteases produced by fungi are normally an important part of the pathogenic process in the host. We examined the effect of different carbon sources - pectin, glucose, and cell wall of P. vulgaris on the production of proteases in cultures of S. sclerotiorum. These proteases were also assayed in infected P. vulgaris plants. Enzyme activity was increased with all carbon sources, but the highest levels were found when pectin was added. Based on real- time quantitative reverse transcription-PCR analyses, protease induction in S. sclerotiorum was found to occur at the level of gene transcription. The finding of increased expression of acid phosphatase 1 and aspartyl protease in vivo in infected P. vulgaris plants supports the role of these enzymes in the invasion process of S. sclerotiorum.
Palavras-chave: Protease
asp5
Phaseolus vulgaris
acp1
Sclerotinia sclerotiorum
White mold
País: Brasil
Unidade acadêmica: Instituto de Ciências Biológicas - ICB (RG)
Citação: BUENO, E. A. et al. Effect of different carbon sources on proteases secreted by the fungal pathogen Sclerotinia sclerotiorum during Phaseolus vulgaris infection. Genetics and Molecular Research, Ribeirão Preto, v. 11, n. 2, p. 2171-2181, 2012.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.4238/2012.June.25.3.
Identificador do documento: 10.4238/2012.June.25.3.
URI: http://repositorio.bc.ufg.br/handle/ri/16229
Data de publicação: 2012
Aparece nas coleções:ICB - Artigos publicados em periódicos

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