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Campo DCValorIdioma
dc.creatorBueno, E. A.-
dc.creatorOliveira, Marília Barros-
dc.creatorAndrade, Rosangela Vieira de-
dc.creatorLobo Junior, Murillo -
dc.creatorSilva, Silvana Petrofeza da-
dc.date.accessioned2018-10-22T12:37:48Z-
dc.date.available2018-10-22T12:37:48Z-
dc.date.issued2012-
dc.identifier.citationBUENO, E. A. et al. Effect of different carbon sources on proteases secreted by the fungal pathogen Sclerotinia sclerotiorum during Phaseolus vulgaris infection. Genetics and Molecular Research, Ribeirão Preto, v. 11, n. 2, p. 2171-2181, 2012.pt_BR
dc.identifier.issn1676-5680-
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/16229-
dc.description.abstractSclerotinia sclerotiorum (Sclerotiniaceae) is a plant pathogenic fungus that causes white mold disease in vegetable crops, including the common bean (Phaseolus vulgaris). Proteases produced by fungi are normally an important part of the pathogenic process in the host. We examined the effect of different carbon sources - pectin, glucose, and cell wall of P. vulgaris on the production of proteases in cultures of S. sclerotiorum. These proteases were also assayed in infected P. vulgaris plants. Enzyme activity was increased with all carbon sources, but the highest levels were found when pectin was added. Based on real- time quantitative reverse transcription-PCR analyses, protease induction in S. sclerotiorum was found to occur at the level of gene transcription. The finding of increased expression of acid phosphatase 1 and aspartyl protease in vivo in infected P. vulgaris plants supports the role of these enzymes in the invasion process of S. sclerotiorum.pt_BR
dc.language.isoengpt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectProteasept_BR
dc.subjectasp5pt_BR
dc.subjectPhaseolus vulgarispt_BR
dc.subjectacp1pt_BR
dc.subjectSclerotinia sclerotiorumpt_BR
dc.subjectWhite moldpt_BR
dc.titleEffect of different carbon sources on proteases secreted by the fungal pathogen Sclerotinia sclerotiorum during Phaseolus vulgaris infectionpt_BR
dc.typeArtigopt_BR
dc.publisher.countryBrasilpt_BR
dc.identifier.doi10.4238/2012.June.25.3.-
dc.publisher.departmentInstituto de Ciências Biológicas - ICB (RG)pt_BR
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