Analysis of genes that are differentially expressed during the Sclerotinia sclerotiorum–Phaseolus vulgaris interaction
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Data
2015-10
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Resumo
The fungus Sclerotinia sclerotiorum (Lib.) de Bary, one of the most important plant
pathogens, causes white mold on a wide range of crops. Crop yield can be dramatically
decreased due to this disease, depending on the plant cultivar and environmental
conditions. In this study, a suppression subtractive hybridization cDNA library approach
was used for the identification of pathogen and plant genes that were differentially
expressed during infection of the susceptible cultivar BRS Pérola of Phaseolus vulgaris
L. A total of 979 unigenes (430 contigs and 549 singletons) were obtained and
classified according to their functional categories. The transcriptional profile of 11 fungal
genes related to pathogenicity and virulence were evaluated by reverse transcription
quantitative real-time polymerase chain reaction (RT-qPCR). Additionally, the temporal
expression profile obtained by RT-qPCR was evaluated for the following categories of
plant defense-related genes: pathogenesis-related genes (PvPR1, PvPR2, and PvPR3),
phenylpropanoid pathway genes (PvIsof, PvFPS1, and 4CL), and genes involved in
defense and stress-related categories (PvLox, PvHiprp, PvGST, PvPod, and PvDox).
Data obtained in this study provide a starting point for achieving a better understanding
of the pathosystem S. sclerotiorum–P. vulgaris.
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Palavras-chave
Necrotrophic fungi, Stress-induced transcripts, Common bean, Sclerotinia sclerotiorum, Suppression subtractive hybridization, Gene expression
Citação
OLIVEIRA, Marília B. et al. Analysis of genes that are differentially expressed during the Sclerotinia sclerotiorum-Phaseolus vulgaris interaction. Frontiers in Microbiology, Lausanne, v. 6, p. 1162, Oct. 2015.