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Tipo do documento: Artigo
Título: Host protein biomarkers identify active tuberculosis in HIV uninfected and co-infected individuals
Autor: Achkar, Jacqueline M.
Cortes, Laetitia
Croteau, Pascal
Yanofsky, Corey
Mentinova, Marija
Rajotte, Isabelle
Schirm, Michael
Zhouc, Yiyong
Kipnis, Ana Paula Junqueira
Kasprowicz, Victoria O.
Larsen, Michelle
Allard, René
Hunter, Joanna
Paramithiotis, Eustache
Abstract: Biomarkers for active tuberculosis (TB) are urgently needed to improve rapid TB diagnosis. The objective of this study was to identify serum protein expression changes associated with TB but not latent Mycobacterium tuberculosis infection (LTBI), uninfected states, or respiratory diseases other than TB (ORD). Serum samples from 209 HIV uninfected (HIV−) and co-infected (HIV+) individuals were studied. In the discovery phase samples were analyzed via liquid chromatography and mass spectrometry, and in the verification phase biologically independent samples were analyzed via a multiplex multiple reaction monitoring mass spectrometry (MRM-MS) assay. Compared to LTBI and ORD, host proteins were significantly differentially expressed in TB, and involved in the immune response, tissue repair, and lipid metabolism. Biomarker panels whose composition differed according to HIV status, and consisted of 8 host proteins in HIV− individuals (CD14, SEPP1, SELL, TNXB, LUM, PEPD, QSOX1, COMP, APOC1), or 10 host proteins in HIV+ individuals (CD14, SEPP1, PGLYRP2, PFN1, VASN, CPN2, TAGLN2, IGFBP6), respectively, distinguished TB from ORD with excellent accuracy (AUC=0.96 for HIV− TB, 0.95 for HIV+ TB). These results warrant validation in larger studies but provide promise that host protein biomarkers could be the basis for a rapid, blood-based test for TB.
Palavras-chave: Host proteins
País: Holanda
Unidade acadêmica: Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
Citação: ACHKAR, Jacqueline M. Host protein biomarkers identify active tuberculosis in HIV uninfected and co-infected individuals. EBioMedicine, Amsterdã, v. 2, p. 1160-1168, 2015.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1016/j.ebiom.2015.07.039
Identificador do documento: 10.1016/j.ebiom.2015.07.039
Data de publicação: 2015
Aparece nas coleções:IPTSP - Artigos publicados em periódicos

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