Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/16348
Tipo do documento: Artigo
Título: Detection of selected bacterial species in intraoral sites of patients with chronic periodontitis using multiplex polymerase chain reaction
Autor: Estrela, Cyntia Rodrigues de Araújo
Pimenta, Fabiana Cristina
Alencar, Ana Helena Gonçalves de
Ruiz, Luís Fernando Naldi
Estrela, Carlos
Abstract: Objective: The aim of this study was to detect the prevalence of selected bacterial species in intraoral sites of patients with chronic periodontitis (CP) using multiplex polymerase chain reaction (PCR). Methodology: Samples were collected from the tongue dorsum, buccal mucosa, supragingival and subgingival plaque and saliva of 30 patients with untreated CP. Multiplex PCR was used to determine prevalence rates, which were then compared using a chi-square test. Significance level was set at p<0.05. Mean and standard deviation values were used to evaluate variations in prevalence according to site. Results: The prevalence of S. mutans was 70% in saliva; 60% in samples collected from the tongue dorsum; 50% in samples collected from the buccal mucosa; 56.5% in the supragingival plaque; and 53.5% in the subgingival plaque. The prevalence of E. faecalis ranged from 3.5% to 13.5% in all intraoral microenvironment. The highest prevalence of P. gingivalis was found in subgingival plaque (53.5%), and of P. intermedia in supragingival plaque (33.5%), subgingival plaque (30%) and tongue dorsum (33.5%). The prevalence of bacteria did not vary significantly among the intraoral sites. Conclusions: All studied bacteria were identified in intraoral sites. S. mutans, P. gingivalis and P. intermedia had high prevalence rates, but the prevalence of E. faecalis was low. Multiplex PCR proved to be an adequate method for epidemiological studies.
Palavras-chave: Chronic periodontitis
Epidemiological studies
Bacteria
DNA probes
PCR
País: Brasil
Unidade acadêmica: Faculdade de Odontologia - FO (RG)
Citação: ESTRELA, Cyntia Rodrigues de Araújo et al. Detection of selected bacterial species in intraoral sites of patients with chronic periodontitis using multiplex polymerase chain reaction. Journal of Applied Oral Science, Bauru, v. 18, n. 4, p. 423, 2010.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1590/S1678-77572010000400018
Identificador do documento: 10.1590/S1678-77572010000400018
URI: http://repositorio.bc.ufg.br/handle/ri/16348
Data de publicação: 2010
Aparece nas coleções:FM - Artigos publicados em periódicos

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