Detection of organochlorine compounds formed during the contact of sodium hypochlorite with dentin and dental pulp
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Data
2014
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Resumo
This study used gas chromatography-mass spectrometry (GC-MS) to detect the products
formed during the contact of sodium hypochlorite (NaOCl) with bovine pulp and
dentin. For analysis of the products formed in the volatile phase, 11 mg of bovine pulp
tissue were placed in contact with 0.5%, 2.5% and 5.25% NaOCl until complete tissue
dissolution occurred. The solid phase microextraction (SPME) fiber was exposed inside
the container through the cover membrane and immediately injected into the GC-MS
system. 30 mg of the of dentin were kept in contact with NaOCl, and then the SPME
fiber was exposed inside the container through the cover membrane for adsorption of
the products and injected into the GC-MS system. The same protocol was used for the
aqueous phase. For analysis of the volatile compounds, the final solution was extracted
using pure ethyl ether. The suspended particulate phase of the mixture was aspirated,
and ether was separated from the aqueous phase of the solution. The ether containing
the products that resulted from the chemical interaction of dentin and pulp with the
NaOCl was filtered and then injected into the GC-MS system for analysis of the aqueous
phase. The aqueous and volatile phases of both dentin and pulp showed the formation of
chloroform, hexachloroethane, dichloromethylbenzene and benzaldehyde. In conclusion,
organochlorine compounds are generated during the contact of dentin and pulp with
NaOCl at concentrations of 0.5%, 2.5% and 5.25%.
Descrição
Palavras-chave
Sodium hypochlorite, Organochlorine, Chloroform, Hexachloroethan, Dichloromethylbenzene
Citação
VARISE, Tiago Gilioli et al. Detection of organochlorine compounds formed during the contact of sodium hypochlorite with dentin and dental pulp. Brazilian Dental Journal, Ribeirão Preto, v. 25, n. 2, p. 109-116, 2014.