Antimicrobial analysis of different root canal filling pastes used in pediatric dentistry by two experimental methods
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Data
2006
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Resumo
The objective of this study was to compare, by two experimental methods, the antimicrobial efficacy of different root canal filling
pastes used in pediatric dentistry. The tested materials were: Guedes-Pinto paste (GPP), zinc oxide-eugenol paste (OZEP), calcium
hydroxide paste (CHP), chloramphenicol + tetracycline + zinc oxide and eugenol paste (CTZP) and Vitapex ® . Fiven microbial strains
(S. aureus, E. faecalis, P. aeruginosa, B. subtilis and C. albicans) obtained from the American Type Culture Collection were inoculated
in Brain Heart Infusion (BHI) and incubated at 37oC for 24 h. For the direct exposure test (DET), 72 paper points were contaminated
with the standard microbial suspensions and exposed to the root canal filling pastes for 1, 24, 48 and 72 h. The points were immersed
in Letheen Broth (LB), followed by incubation at 37oC for 48 h. An inoculum of 0.1 mL obtained from LB was then transferred to 7 mL
of BHI, under identical incubations conditions and the microbial growth was evaluated. The pastes showed activity between 1 and 24
h, depending on the material. For the agar diffusion test (ADT), 30 Petri plates with 20 mL of BHI agar were inoculated with 0.1 mL
of the microbial suspension, using sterile swabs that were spread on the medium. Three cavities were made in each agar plate (total =
90) and completely filled with one of the filling root canal pastes. The plates were pre-incubated for 1 h at room temperature and then
incubated at 37oC for 24 to 48 h. The inhibition zone around each well was recorded in mm. The complete antimicrobial effect in the
direct exposure test was observed after 24 h on all microbial indicators. All root canal filling materials induced the formation of inhibition
zones, except for Vitapex ® (range, 6.0-39.0 mm).
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Calcium hydroxide, Intracanal dressing, Pediatric dentistry
Citação
AMORIM, Lilian de Fátima Guedes de et al. Antimicrobial analysis of different root canal filling pastes used in pediatric dentistry by two experimental methods. Brazilian Dental Journal, Ribeirão Preto, v. 17, n. 4, p. 75-81, 2006.