Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/16711
Tipo do documento: Artigo
Título: Evaluation of a commercial real-time PCR kit for detection of dengue virus in samples collected during an outbreak in Goiania, central Brazil, in 2005
Autor: Levi, José Eduardo
Tateno, Adriana Fumie
Machado, Adriana Freire
Ramalho, Débora Camillo
Souza, Vanda Akico Ueda Fick de
Guilarde, Adriana Oliveira
Feres, Valéria Christina de Rezende
Martelli, Celina Maria Turchi
Turchi, Marília Dalva
Siqueira Júnior, João Bosco
Pannuti, Cláudio Sérgio
Abstract: In the past 2 decades, dengue has reemerged in Brazil as a significant public health problem. Clinicians demand a diagnostic test with high sensitivity that is applicable during the early symptomatic phase. We aimed to test two distinct molecular methods on samples from suspected dengue cases during an outbreak in Central Brazil. Acute-phase serum specimens from 254 patients suspected of having dengue were collected during 2005 in the city of Goiaˆnia, Central Brazil. Samples were blindly evaluated by real-time and multiplex PCR in addition to routine immunoglobulin M serology and virus culture. Overall, acute dengue was confirmed by serology, multiplex PCR, or virus isolation for 80% of patients (203/254). Another four patients presented real-time PCR-positive results as the unique marker of dengue. Higher real-time PCR positivity levels and viral loads were observed in the early symptomatic phase of disease (<5 days) than after this period. Multiplex and real-time PCR assays presented a high kappa agreement (0.85). According to multiplex PCR, 60 samples harbored dengue virus type 3 (DEN-3), 4 samples harbored DEN-2, and 1 sample displayed a pattern compatible with a double infection with DEN-2 and -3. The dengue virus real-time kit was found to be practical and adjustable for high throughput, to display the best performance in the early symptomatic phase of dengue cases, and to be valuable for confirming dengue diagnosis in a timely manner.
País: Estados unidos
Unidade acadêmica: Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
Citação: LEVI, José Eduardo et al. Evaluation of a commercial real-time PCR kit for detection of dengue virus in samples collected during an outbreak in Goiania, central Brazil, in 2005. Journal of Clinical Microbiology, Washington, v. 45, n. 6, p. 1893-1897, 2007.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1128/JCM.00065-07
Identificador do documento: 10.1128/JCM.00065-07
URI: http://repositorio.bc.ufg.br/handle/ri/16711
Data de publicação: Jun-2007
Aparece nas coleções:IPTSP - Artigos publicados em periódicos

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