In vitro suscetibility testing of dermatophytes isolated in Goiania, Brazil, against five antifungal agents by broth microdilution method

Resumo

The antifungal activities of fluconazole, itraconazole, ketoconazole, terbinafine and griseofulvin were tested by broth microdilution technique, against 60 dermatophytes isolated from nail or skin specimens from Goiania city patients, Brazil. In this study, the microtiter plates were incubated at 28 oC allowing a reading of the minimal inhibitory concentration (MIC) after four days of incubation for Trichophyton mentagrophytes and five days for T. rubrum and Microsporum canis. Most of the dermatophytes had uniform patterns of susceptibility to the antifungal agents tested. Low MIC values as 0.03 µg/mL were found for 33.3%, 31.6% and 15% of isolates for itraconazole, ketoconazole and terbinafine, respectively.

Descrição

Palavras-chave

Minimal inhibitory concentration, Dermatophytes, Broth microdilution method

Citação

ARAÚJO, Crystiane Rodrigues de; MIRANDA, Karla Carvalho; FERNANDES, Orionalda de Fatima Lisboa; SOARES, Ailton José; SILVA, Maria do Rosário Rodrigues. In vitro suscetibility testing of dermatophytes isolated in Goiania, Brazil, against five antifungal agents by broth microdilution method. Revista do Instituto de Medicina Tropical de São Paulo, São Paulo, v. 51, n. 1, p. 9-12, 2009.