Use este identificador para citar ou linkar para este item:
Tipo do documento: Artigo
Título: Structural and functional characterization of a multifunctional alanine-rich peptide analogue from Pleuronectes americanus
Autor: Migliolo, Ludovico
Silva, Osmar Nascimento
Silva, Paula Andreia
Reis, Maysa Paula da Costa
Costa, Carolina Rodrigues
Silva, Diego Oliveira Nolasco da
Barbosa, João Alexandre Ribeiro Gonçalves
Silva, Maria do Rosário Rodrigues
Bemquerer, Marcelo Porto
Lima, Lídia Maria Pinto de
Romanos, Maria Teresa Villela
Freitas, Sonia Maria de
Magalhães, Beatriz Simas
Franco, Octavio Luiz
Abstract: Recently, defense peptides that are able to act against several targets have been characterized. The present work focuses on structural and functional evaluation of the peptide analogue Pa-MAP, previously isolated as an antifreeze peptide from Pleuronectes americanus. Pa-MAP showed activities against different targets such as tumoral cells in culture (CACO-2, MCF-7 and HCT-116), bacteria (Escherichia coli ATCC 8739 and Staphylococcus aureus ATCC 25923), viruses (HSV-1 and HSV-2) and fungi (Candida parapsilosis ATCC 22019, Trichophyton mentagrophytes (28d&E) and T. rubrum (327)). This peptide did not show toxicity against mammalian cells such as erythrocytes, Vero and RAW 264.7 cells. Molecular mechanism of action was related to hydrophobic residues, since only the terminal amino group is charged at pH 7 as confirmed by potentiometric titration. In order to shed some light on its structure-function relations, in vitro and in silico assays were carried out using circular dichroism and molecular dynamics. Furthermore, Pa-MAP showed partial unfolding of the peptide changes in a wide pH (3 to 11) and temperature (25 to 95uC) ranges, although it might not reach complete unfolding at 95uC, suggesting a high conformational stability. This peptide also showed a conformational transition with a partial a-helical fold in water and a full a-helical core in SDS and TFE environments. These results were corroborated by spectral data measured at 222 nm and by 50 ns dynamic simulation. In conclusion, data reported here show that Pa-MAP is a potential candidate for drug design against pathogenic microorganisms due to its structural stability and wide activity against a range of targets.
País: Estados unidos
Unidade acadêmica: Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
Citação: MIGLIOLO, Ludovico et al. Structural and functional characterization of a multifunctional alanine-rich peptide analogue from Pleuronectes americanus. Plos One, San Francisco, v. 7, n. 10, e4707, 2012.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1371/journal.pone.0047047
Identificador do documento: 10.1371/journal.pone.0047047
Data de publicação: 8-Out-2012
Aparece nas coleções:IPTSP - Artigos publicados em periódicos

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
Artigo - Ludovico Migliolo - 2012.pdf606,71 kBAdobe PDFThumbnail

Este item está licenciada sob uma Licença Creative Commons Creative Commons