Parasitismo do encéfalo de ratos por Trypanosoma cruzi
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Data
2010-06
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Editor
Ricardo Menegatti
Resumo
Chagas made the fist report on encephalic parasitism in chagasic patients. The greater susceptibility
of the children has been established. This present work aims at assessing the encephalic parasitism
in 10-day-old and 30-day-old Holtzman rats after infection whit low and high number of
trypomastigotes of Trypanosoma cruzi. Male Holtzman rats whit 10-day-old and 30-day-old were inoculates whit 5 x 103 or 2,5 x 104 tripomastigotes of the Y strain of T. cruzi intraperitoneally. The
animals were killed 13 days after inoculation under chloral hydrate perfused whit 4% phosphatebuffered
paraformaldehyde. The fragments were processed for paraplast or glycolmethacrylate
embedding. The paraplast sections were stained whit hematoxilina-eosin and resin section whit
toluidine blue-orange C-erytrosin. Immunohistochemical were performed by using anti-T.cruzi serum
obtained from T. cruzi-infected rabbit and a peroxidase conjugated-anti-rabbit IgG (Sigma). The
encephalic parasitism was discrete in the infection whit 5 x 103 both 10-day-old and 30-day-old
being revealed by sparse glial nodules. The inoculation of the 2,5 x 104 tripomastigotes/50g of body
weight induced more intense encephalic parasitism in the group infected at day 10 of age.
Encephalic areas devoid of blood brain barrier showed very low parasitism.
Descrição
Palavras-chave
Encéfalo, Trypanosoma cruzi, Ratos, Brain, Trypanosoma cruzi, Rats
Citação
MATA, João Roberto da; SANTOS, Sara Dayane; MATA, Fabiana Ribeiro da. Parasitismo do encéfalo de ratos por Trypanosoma cruzi. Revista Eletrônica de Farmácia, Goiânia, v. 7, n. 2, p. 70-77, abr./jun. 2010. Disponível em: <https://revistas.ufg.br/REF/article/view/11863/7805>.