Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/16895
Tipo do documento: Artigo
Título: Comparison of two rapid tests for anti-phenolic glycolipid-I serology in Brazil and Nepal
Autor: Stefani, Mariane Martins de Araújo
Grassi, Adriano Badotti
Sampaio, Lucas Henrique
Sousa, Ana Lúcia Osório Maroclo de
Costa, Maurício Barcelos
Scheelbeek, Pauline
Neupane, Kapil D.
Hagge, Deanna A.
Macdonald, Murdo
Sang-Nae, Cho
Oskam, Linda
Bührer-Sékula, Samira
Abstract: The diagnosis of leprosy continues to be based on clinical symptoms and early diagnosis and treatment are criti-cal to preventing disability and transmission. Sensitive and specific laboratory tests are not available for diagnosing leprosy. Despite the limited applicability of anti-phenolic glycolipid-I (PGL-I) serology for diagnosis, it has been suggested as an additional tool to classify leprosy patients (LPs) for treatment purposes. Two formats of rapid tests to detect anti-PGL-I antibodies [ML immunochromatography assay (ICA) and ML Flow] were compared in differ-ent groups, multibacillary patients, paucibacillary patients, household contacts and healthy controls in Brazil and Nepal. High ML Flow intra-test concordance was observed and low to moderate agreement between the results of ML ICA and ML Flow tests on the serum of LPs was observed. LPs were “seroclassified” according to the results of these tests and the seroclassification was compared to other currently used classification systems: the World Health Organization operational classification, the bacilloscopic index and the Ridley-Jopling classification. When analysing the usefulness of these tests in the operational classification of PB and MB leprosy for treatment and follow-up purposes, the ML Flow test was the best point-of-care test for subjects in Nepal and despite the need for sample dilution, the ML ICA test yielded better performance among Brazilian subjects. Our results identified pos-sible ways to improve the performance of both tests.
Palavras-chave: Immunochromatography
Leprosy
Serologic tests
País: Brasil
Unidade acadêmica: Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
Citação: STEFANI, Mariane Martins de Araújo et al. Comparison of two rapid tests for anti phenolic glycolipid-I serology in Brazil and Nepal. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 107, suppl. 1, p. 124-131, 2012.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1590/S0074-02762012000900019
Identificador do documento: 10.1590/S0074-02762012000900019
URI: http://repositorio.bc.ufg.br/handle/ri/16895
Data de publicação: 2012
Aparece nas coleções:IPTSP - Artigos publicados em periódicos

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