Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/16984
Tipo do documento: Artigo
Título: Trypanosoma cruzi infection and/or administration of the nonsteroidal anti-inflammatory nimesulide increase the number of colonic crypts overexpressing metallothioneins in rat colon carcinogenesis
Autor: Escalante, Rodrigo Dornfeld
Oliveira, Enio Chaves de
Cunha, F. Q.
Vespúcio, Marcelo Vinícius Oliveira
Silva, Alfredo Ribeiro da
Aprilli, Francisco
Garcia, S. B.
Abstract: Trypanosoma cruzi infection and nonsteroidal anti-inflammatory drugs inhibit colorectal carcinogenesis by mechanisms not completely known and metallothionein proteins (MTs) may be involved in this process. Sixty-six male Wistar rats weighing 90 to 120 g were randomly divided into seven groups (GI to GVII). GI, GII and GIII animals were subcutaneously infected with 200,000 trypomastigote forms of the Y strain of T. cruzi. After 8 weeks, GI, GII, GIV, and GVI were injected with one weekly subcutaneous dose of 12 mg/kg dimethylhydrazine for 4 weeks. In sequence, GI, GIV and GV were treated with nimesulide (10 mg/kg per dose, five times per week for 8 weeks). Groups I, III, IV, and VI had 12 animals, and each of the other groups had 6 animals. All the animals were euthanized 8 weeks after the last dimethylhydrazine injection. The colons were fixed and processed for MT immunohisto- chemistry. The index of MT-overexpressing colonic crypts (MTEC) was estimated as the percentage of MT-stained crypts in relation to the total number of crypts scored. Five hundred crypts per animal were scored. Data were analyzed by the Kruskal-Wallis test followed by the Dunn test. There was an increase in MTEC index in the groups either infected with T. cruzi or treated with nimesulide or both infected and treated when compared to control (401, 809, and 1011%, respective- ly). We suggest that the increased formation of MTEC may be related to the protection against carcinogenesis provided both by T. cruzi infection and nimesulide.
Palavras-chave: Trypanosoma cruzi
Dimethylhydrazine
Metallothioneins
Colon carcinogenesis
Nimesulide
País: Brasil
Unidade acadêmica: Faculdade de Medicina - FM (RG)
Citação: ESCALANTE, R. D. et al. Trypanosoma cruzi infection and/or administration of the nonsteroidal anti-inflammatory nimesulide increase the number of colonic crypts overexpressing metallothioneins in rat colon carcinogenesis. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 39, n. 7, p. 895-899, 2006.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1590/S0100-879X2006000700007
Identificador do documento: 10.1590/S0100-879X2006000700007
URI: http://repositorio.bc.ufg.br/handle/ri/16984
Data de publicação: 2006
Aparece nas coleções:FM - Artigos publicados em periódicos

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
Artigo - Rodrigo Dornfeld Escalante - 2006.pdf53,78 kBAdobe PDFThumbnail
Baixar/Abrir


Este item está licenciada sob uma Licença Creative Commons Creative Commons