Cellular proliferation in the gills of guppies exposed to pequi ethanolic extracts
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Data
2014-06
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Editor
Vera Lúcia Gomes Klein
Resumo
The bioactive principles present in the ethanolic extract of “pequi” (Caryocar brasiliensis)
have proven to be molluscicidal, fungicidal, trypanocidal, insecticide, and leshimanicidal, taking potential
use to combat aquatic pests and vectors in public health, and for infectious and invasive diseases in fish.
Many plants have proven moluscidida and insecticide effects, and its extracts may contribute to vector
control in public health. They also have prophylactic and therapeutic effects in combating diseases in
fish, for ornamental and aquaculture systems. Most of them are commonly found in tropical and subtropical
countries. However, to be released in environment or used for health and/or combating invasive
diseases in fish, the effect of these products on other representatives of aquatic fauna must be better
understood. The guppy (Poecilia vivipara) is generally used as a bioindicator of environmental toxicity,
then, he was elected to the experimental studies carried out to verify the action of these extracts. The
gills are the target organ, as they come into direct contact with water and because its morphophysiological
activity in demonstrate the effects of substances present in the water. Therefore, the extracts
obtained from the stem and leaf of pequi were analyzed chromatographically, and then the extracts rich
in flavonoids were selected. The effects in acute tests of these extracts were verified histologically and
immunohistochemically using the molecular marker BrdU (5-bromo-2’-deoxyuridine) that binds to cellular
DNA during the S phase of their cycle. This work confirms the potential action of “pequi” as inducer
of cell proliferation in gills of fish. As the test was conducted in a short period of time and was proven
the occurrence of cell proliferation exist the possibility of having future tumor formation, which indicate
a need for studies with long-term exposures.
Descrição
Palavras-chave
Compostos fitoquímicos, Extrato etanólico, Cromatografia, Imunohistoquimica, Proliferação celular, Phytochemical compounds, Ethanolic extract, Chromatography, Immunohistochemistry, Cell proliferation
Citação
VIEIRA, Eliane Rosa; SANTOS, Suzana da Costa; DIAS JUNIOR, Walter; SABÓIAS-MORAIS, Simone Maria Teixeira. Cellular proliferation in the gills of guppies exposed to pequi ethanolic extracts. Revista de Biologia Neotropical, Goiânia, v. 11, n. 1, p. 58-70, jan./jun. 2014. Disponível em: <https://www.revistas.ufg.br/RBN/article/view/22711/18092>.