Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/17039
Tipo do documento: Artigo
Título: Development of a quantitative rapid diagnostic test for multibacillary leprosy using smart phone technology
Autor: Cardoso, Ludimila Paula Vaz
Dias, Ronaldo Ferreira
Freitas, Aline Araújo
Hungria, Emerith Mayra
Oliveira, Regiane Morillas
Collovati, Marco
Reed, Steven G.
Duthie, Malcolm S.
Stefani, Mariane Martins Araújo
Abstract: Background: Despite efforts to eliminate leprosy as public health problem, delayed diagnosis and disabilities still occur in many countries. Leprosy diagnosis remains based on clinical manifestations and the number of clinicians with expertise in leprosy diagnosis is in decline. We have developed a new immunochromatographic test with the goal of producing a simple and rapid system that can be used, with a minimal amount of training, to provide an objective and consistent diagnosis of multibacillary leprosy. Methods: The test immobilizes two antigens that have been recognized as excellent candidates for serologic diagnosis (the PGL-I mimetic, ND-O, and LID-1), on a nitrocellulose membrane. This allows the detection of specific IgM and IgG antibodies within 20 minutes of the addition of patient sera. Furthermore, we coupled the NDO-LID® rapid tests with a new cell phone-based test reader platform (Smart Reader®) to provide objective interpretation that was both quantifiable and consistent. Results: Direct comparison of serologic responses indicated that the rapid test detected a greater proportion of leprosy patients than a lab-based PGL-I ELISA. While positive responses were detected by PGL-I ELISA in 83.3% of multibacillary patients and 15.4% of paucibacillary patients, these numbers were increased to 87% and 21.2%, respectively, when a combination of the NDO-LID® test and Smart Reader® was used. Among multibacillary leprosy the sensitivity of NDO-LID® test assessed by Smart Reader® was 87% (95% CI, 79.2-92.7%) and the specificity was 96.1% (95% CI, 91.7- 98.6%). The positive predictive value and the negative predictive value of NDO-LID® tests were 94% (95% CI, 87.4-97.8%) and 91.4% (95% CI, 85.9-95.2%), respectively. Conclusion: The widespread provision of rapid diagnostic tests to facilitate the diagnosis or prognosis of multibacillary leprosy could impact on leprosy control programs by aiding early detection, directing appropriate treatment and potentially interrupting Mycobacterium leprae transmission.
Palavras-chave: Brazil
Leprosy
Serologic response
Lateral flow
País: Gra-bretanha
Unidade acadêmica: Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
Citação: CARDOSO, Ludimila Paula Vaz et al. Development of a quantitative rapid diagnostic test for multibacillary leprosy using smart phone technology. BMC Infectious Diseases, London, v. 13, n. 497, 2013.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1186/1471-2334-13-497
Identificador do documento: 10.1186/1471-2334-13-497
URI: http://repositorio.bc.ufg.br/handle/ri/17039
Data de publicação: 2013
Aparece nas coleções:IPTSP - Artigos publicados em periódicos

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